Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain

Research output: Contribution to journal › Journal article › Research › peer-review

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Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain. / Schou-Pedersen, Anne Marie Voigt; Hansen, Stine Normann; Tveden-Nyborg, Pernille; Lykkesfeldt, Jens.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 1028, 15.08.2016, p. 222-230.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schou-Pedersen, AMV, Hansen, SN, Tveden-Nyborg, P & Lykkesfeldt, J 2016, 'Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 1028, pp. 222-230. https://doi.org/10.1016/j.jchromb.2016.05.048

APA

Schou-Pedersen, A. M. V., Hansen, S. N., Tveden-Nyborg, P., & Lykkesfeldt, J. (2016). Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1028, 222-230. https://doi.org/10.1016/j.jchromb.2016.05.048

Vancouver

Schou-Pedersen AMV, Hansen SN, Tveden-Nyborg P, Lykkesfeldt J. Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2016 Aug 15;1028:222-230. https://doi.org/10.1016/j.jchromb.2016.05.048

Author

Schou-Pedersen, Anne Marie Voigt ; Hansen, Stine Normann ; Tveden-Nyborg, Pernille ; Lykkesfeldt, Jens. / Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2016 ; Vol. 1028. pp. 222-230.

Bibtex

@article{2dc9c7b1b5fc4a81af24a766acef9a56,
title = "Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain",
abstract = "In the present paper, we describe a validated chromatographic method for the simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell culture and in sub-regions of the guinea pig brain. Electrochemical detection provided limits of quantifications (LOQs) between 3.6 and 12 nM. Within the linear range, obtained recoveries were from 90.9 ± 9.9 to 120 ± 14% and intra-day and inter-day precisions found to be less than 5.5% and 12%, respectively. The analytical method was applicable for quantification of intracellular and extracellular amounts of monoamine neurotransmitters and their metabolites in guinea pig frontal cortex and hippocampal primary neuronal cell cultures. Noradrenaline, dopamine and serotonin were found to be in a range from 0.31 to 1.7 pmol per 2 million cells intracellularly, but only the biogenic metabolites could be detected extracellularly. Distinct differences in monoamine concentrations were observed when comparing concentrations in guinea pig frontal cortex and cerebellum tissue with higher amounts of dopamine and its metabolites, 3,4-dihydroxyphenylacetic acid and homovanillic acid in frontal cortex, as compared to cerebellum. The chemical turnover in frontal cortex tissue of guinea pig was for serotonin successfully predicted from the turnover observed in the frontal cortex cell culture. In conclusion, the present analytical method shows high precision, accuracy and sensitivity and is broadly applicable to monoamine measurements in cell cultures as well as brain biopsies from animal models used in preclinical neurochemistry.",
keywords = "Faculty of Health and Medical Sciences, Monoamines, HPLC, Electrochemical detection, Cell culture, Guinea pig",
author = "Schou-Pedersen, {Anne Marie Voigt} and Hansen, {Stine Normann} and Pernille Tveden-Nyborg and Jens Lykkesfeldt",
year = "2016",
month = aug,
day = "15",
doi = "10.1016/j.jchromb.2016.05.048",
language = "English",
volume = "1028",
pages = "222--230",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell cultures and in sub-regions of guinea pig brain

AU - Schou-Pedersen, Anne Marie Voigt

AU - Hansen, Stine Normann

AU - Tveden-Nyborg, Pernille

AU - Lykkesfeldt, Jens

PY - 2016/8/15

Y1 - 2016/8/15

N2 - In the present paper, we describe a validated chromatographic method for the simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell culture and in sub-regions of the guinea pig brain. Electrochemical detection provided limits of quantifications (LOQs) between 3.6 and 12 nM. Within the linear range, obtained recoveries were from 90.9 ± 9.9 to 120 ± 14% and intra-day and inter-day precisions found to be less than 5.5% and 12%, respectively. The analytical method was applicable for quantification of intracellular and extracellular amounts of monoamine neurotransmitters and their metabolites in guinea pig frontal cortex and hippocampal primary neuronal cell cultures. Noradrenaline, dopamine and serotonin were found to be in a range from 0.31 to 1.7 pmol per 2 million cells intracellularly, but only the biogenic metabolites could be detected extracellularly. Distinct differences in monoamine concentrations were observed when comparing concentrations in guinea pig frontal cortex and cerebellum tissue with higher amounts of dopamine and its metabolites, 3,4-dihydroxyphenylacetic acid and homovanillic acid in frontal cortex, as compared to cerebellum. The chemical turnover in frontal cortex tissue of guinea pig was for serotonin successfully predicted from the turnover observed in the frontal cortex cell culture. In conclusion, the present analytical method shows high precision, accuracy and sensitivity and is broadly applicable to monoamine measurements in cell cultures as well as brain biopsies from animal models used in preclinical neurochemistry.

AB - In the present paper, we describe a validated chromatographic method for the simultaneous quantification of monoamine neurotransmitters and their biogenic metabolites intracellularly and extracellularly in primary neuronal cell culture and in sub-regions of the guinea pig brain. Electrochemical detection provided limits of quantifications (LOQs) between 3.6 and 12 nM. Within the linear range, obtained recoveries were from 90.9 ± 9.9 to 120 ± 14% and intra-day and inter-day precisions found to be less than 5.5% and 12%, respectively. The analytical method was applicable for quantification of intracellular and extracellular amounts of monoamine neurotransmitters and their metabolites in guinea pig frontal cortex and hippocampal primary neuronal cell cultures. Noradrenaline, dopamine and serotonin were found to be in a range from 0.31 to 1.7 pmol per 2 million cells intracellularly, but only the biogenic metabolites could be detected extracellularly. Distinct differences in monoamine concentrations were observed when comparing concentrations in guinea pig frontal cortex and cerebellum tissue with higher amounts of dopamine and its metabolites, 3,4-dihydroxyphenylacetic acid and homovanillic acid in frontal cortex, as compared to cerebellum. The chemical turnover in frontal cortex tissue of guinea pig was for serotonin successfully predicted from the turnover observed in the frontal cortex cell culture. In conclusion, the present analytical method shows high precision, accuracy and sensitivity and is broadly applicable to monoamine measurements in cell cultures as well as brain biopsies from animal models used in preclinical neurochemistry.

KW - Faculty of Health and Medical Sciences

KW - Monoamines

KW - HPLC

KW - Electrochemical detection

KW - Cell culture

KW - Guinea pig

U2 - 10.1016/j.jchromb.2016.05.048

DO - 10.1016/j.jchromb.2016.05.048

M3 - Journal article

C2 - 27379407

VL - 1028

SP - 222

EP - 230

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

ER -

ID: 163078436