The effect of extracts of Searsia species on epileptiform activity in slices of the mouse cerebral cortex
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The effect of extracts of Searsia species on epileptiform activity in slices of the mouse cerebral cortex. / Pedersen, Mikael Egebjerg; Vestergaard, Henrik Tang; Stafford, Gary Ivan; van Staden, Johannes; Jäger, Anna Katharina.
In: Journal of Ethnopharmacology, Vol. 119, No. 3, 2008, p. 538-541.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The effect of extracts of Searsia species on epileptiform activity in slices of the mouse cerebral cortex
AU - Pedersen, Mikael Egebjerg
AU - Vestergaard, Henrik Tang
AU - Stafford, Gary Ivan
AU - van Staden, Johannes
AU - Jäger, Anna Katharina
N1 - Keywords: Cortical wedge preparation NMDA receptor antagonist Searsia dentata Searsia pyroides Traditional medicine
PY - 2008
Y1 - 2008
N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Searsia dentata and Searsia pyroides are used in traditional South African medicine to treat convulsions and epilepsy. Previous studies have demonstrated that extracts of these plants comprise compounds that bind to the flumazenil-sensitive site on the GABA(A) receptor. However, their use as anticonvulsant medicinal plants cannot be adequately explained by these findings. AIMS: The aim of this study was to examine the possible involvement of the glutamatergic system of extracts from the plants. MATERIALS AND METHODS: The mouse cortical wedge preparation was used for functional characterization of the extracts. The affinity towards the NMDA and the AMPA receptor was investigated using classical [(3)H]-GP39653 and [(3)H]-AMPA binding assays, respectively. RESULTS: The extracts of Searsia dentata and Searsia pyroides inhibited the spontaneous epileptiform discharges in mouse cerebral cortical slices with ED(50) of 0.62 and 1.67mg dry extract/mL, respectively. Both extracts displaced [(3)H]-GP39653 binding and significantly inhibited the NMDA-induced response during co-administration in cortical slices. CONCLUSION: In this study, the NMDA receptor antagonistic effect of the crude ethanolic extracts of these two South African medicinal plants was demonstrated.
AB - ETHNOPHARMACOLOGICAL RELEVANCE: Searsia dentata and Searsia pyroides are used in traditional South African medicine to treat convulsions and epilepsy. Previous studies have demonstrated that extracts of these plants comprise compounds that bind to the flumazenil-sensitive site on the GABA(A) receptor. However, their use as anticonvulsant medicinal plants cannot be adequately explained by these findings. AIMS: The aim of this study was to examine the possible involvement of the glutamatergic system of extracts from the plants. MATERIALS AND METHODS: The mouse cortical wedge preparation was used for functional characterization of the extracts. The affinity towards the NMDA and the AMPA receptor was investigated using classical [(3)H]-GP39653 and [(3)H]-AMPA binding assays, respectively. RESULTS: The extracts of Searsia dentata and Searsia pyroides inhibited the spontaneous epileptiform discharges in mouse cerebral cortical slices with ED(50) of 0.62 and 1.67mg dry extract/mL, respectively. Both extracts displaced [(3)H]-GP39653 binding and significantly inhibited the NMDA-induced response during co-administration in cortical slices. CONCLUSION: In this study, the NMDA receptor antagonistic effect of the crude ethanolic extracts of these two South African medicinal plants was demonstrated.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1016/j.jep.2008.08.009
DO - 10.1016/j.jep.2008.08.009
M3 - Journal article
C2 - 18773951
VL - 119
SP - 538
EP - 541
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
SN - 0378-8741
IS - 3
ER -
ID: 8298001