Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver

Research output: Contribution to journalJournal articlepeer-review

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Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver. / Vestentoft, Peter Siig; Jelnes, Peter; Hopkinson, Branden M; Vainer, Ben; Møllgård, Kjeld; Quistorff, Bjorn; Bisgaard, Hanne C.

In: B M C Developmental Biology, Vol. 11, 2011, p. 56.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Vestentoft, PS, Jelnes, P, Hopkinson, BM, Vainer, B, Møllgård, K, Quistorff, B & Bisgaard, HC 2011, 'Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver', B M C Developmental Biology, vol. 11, pp. 56. https://doi.org/10.1186/1471-213X-11-56, https://doi.org/10.1186/1471-213X-11-56

APA

Vestentoft, P. S., Jelnes, P., Hopkinson, B. M., Vainer, B., Møllgård, K., Quistorff, B., & Bisgaard, H. C. (2011). Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver. B M C Developmental Biology, 11, 56. https://doi.org/10.1186/1471-213X-11-56, https://doi.org/10.1186/1471-213X-11-56

Vancouver

Vestentoft PS, Jelnes P, Hopkinson BM, Vainer B, Møllgård K, Quistorff B et al. Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver. B M C Developmental Biology. 2011;11:56. https://doi.org/10.1186/1471-213X-11-56, https://doi.org/10.1186/1471-213X-11-56

Author

Vestentoft, Peter Siig ; Jelnes, Peter ; Hopkinson, Branden M ; Vainer, Ben ; Møllgård, Kjeld ; Quistorff, Bjorn ; Bisgaard, Hanne C. / Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver. In: B M C Developmental Biology. 2011 ; Vol. 11. pp. 56.

Bibtex

@article{cac28054b82a4005afbbb050bc93af0c,
title = "Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver",
abstract = "ABSTRACT: BACKGROUND: During liver development, intrahepatic bile ducts are thought to arise by a unique asymmetric mode of cholangiocyte tubulogenesis characterized by a series of remodeling stages. Moreover, in liver diseases, cells lining the Canals of Hering can proliferate and generate new hepatic tissue. The aim of this study was to develop protocols for three-dimensional visualization of protein expression, hepatic portal structures and human hepatic cholangiocyte tubulogenesis. RESULTS: Protocols were developed to digitally visualize portal vessel branching and protein expression of hepatic cell lineage and extracellular matrix deposition markers in three dimensions. Samples from human prenatal livers ranging from 7 weeks + 2 days to 15.5 weeks post conception as well as adult normal and acetaminophen intoxicated liver were used. The markers included cytokeratins (CK) 7 and 19, the epithelial cell adhesion molecule (EpCAM), hepatocyte paraffin 1 (HepPar1), sex determining region Y (SRY)-box 9 (SOX9), laminin, nestin, and aquaporin 1 (AQP1). Digital three-dimensional reconstructions using CK19 as a single marker protein disclosed a fine network of CK19 positive cells in the biliary tree in normal liver and in the extensive ductular reactions originating from intrahepatic bile ducts and branching into the parenchyma of the acetaminophen intoxicated liver. In the developing human liver, three-dimensional reconstructions using multiple marker proteins confirmed that the human intrahepatic biliary tree forms through several developmental stages involving an initial transition of primitive hepatocytes into cholangiocytes shaping the ductal plate followed by a process of maturation and remodeling where the intrahepatic biliary tree develops through an asymmetrical form of cholangiocyte tubulogenesis. CONCLUSIONS: The developed protocols provide a novel and sophisticated three-dimensional visualization of vessels and protein expression in human liver during development and disease.",
keywords = "Faculty of Health and Medical Sciences",
author = "Vestentoft, {Peter Siig} and Peter Jelnes and Hopkinson, {Branden M} and Ben Vainer and Kjeld M{\o}llg{\aa}rd and Bjorn Quistorff and Bisgaard, {Hanne C}",
year = "2011",
doi = "10.1186/1471-213X-11-56",
language = "English",
volume = "11",
pages = "56",
journal = "B M C Developmental Biology",
issn = "1471-213X",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver

AU - Vestentoft, Peter Siig

AU - Jelnes, Peter

AU - Hopkinson, Branden M

AU - Vainer, Ben

AU - Møllgård, Kjeld

AU - Quistorff, Bjorn

AU - Bisgaard, Hanne C

PY - 2011

Y1 - 2011

N2 - ABSTRACT: BACKGROUND: During liver development, intrahepatic bile ducts are thought to arise by a unique asymmetric mode of cholangiocyte tubulogenesis characterized by a series of remodeling stages. Moreover, in liver diseases, cells lining the Canals of Hering can proliferate and generate new hepatic tissue. The aim of this study was to develop protocols for three-dimensional visualization of protein expression, hepatic portal structures and human hepatic cholangiocyte tubulogenesis. RESULTS: Protocols were developed to digitally visualize portal vessel branching and protein expression of hepatic cell lineage and extracellular matrix deposition markers in three dimensions. Samples from human prenatal livers ranging from 7 weeks + 2 days to 15.5 weeks post conception as well as adult normal and acetaminophen intoxicated liver were used. The markers included cytokeratins (CK) 7 and 19, the epithelial cell adhesion molecule (EpCAM), hepatocyte paraffin 1 (HepPar1), sex determining region Y (SRY)-box 9 (SOX9), laminin, nestin, and aquaporin 1 (AQP1). Digital three-dimensional reconstructions using CK19 as a single marker protein disclosed a fine network of CK19 positive cells in the biliary tree in normal liver and in the extensive ductular reactions originating from intrahepatic bile ducts and branching into the parenchyma of the acetaminophen intoxicated liver. In the developing human liver, three-dimensional reconstructions using multiple marker proteins confirmed that the human intrahepatic biliary tree forms through several developmental stages involving an initial transition of primitive hepatocytes into cholangiocytes shaping the ductal plate followed by a process of maturation and remodeling where the intrahepatic biliary tree develops through an asymmetrical form of cholangiocyte tubulogenesis. CONCLUSIONS: The developed protocols provide a novel and sophisticated three-dimensional visualization of vessels and protein expression in human liver during development and disease.

AB - ABSTRACT: BACKGROUND: During liver development, intrahepatic bile ducts are thought to arise by a unique asymmetric mode of cholangiocyte tubulogenesis characterized by a series of remodeling stages. Moreover, in liver diseases, cells lining the Canals of Hering can proliferate and generate new hepatic tissue. The aim of this study was to develop protocols for three-dimensional visualization of protein expression, hepatic portal structures and human hepatic cholangiocyte tubulogenesis. RESULTS: Protocols were developed to digitally visualize portal vessel branching and protein expression of hepatic cell lineage and extracellular matrix deposition markers in three dimensions. Samples from human prenatal livers ranging from 7 weeks + 2 days to 15.5 weeks post conception as well as adult normal and acetaminophen intoxicated liver were used. The markers included cytokeratins (CK) 7 and 19, the epithelial cell adhesion molecule (EpCAM), hepatocyte paraffin 1 (HepPar1), sex determining region Y (SRY)-box 9 (SOX9), laminin, nestin, and aquaporin 1 (AQP1). Digital three-dimensional reconstructions using CK19 as a single marker protein disclosed a fine network of CK19 positive cells in the biliary tree in normal liver and in the extensive ductular reactions originating from intrahepatic bile ducts and branching into the parenchyma of the acetaminophen intoxicated liver. In the developing human liver, three-dimensional reconstructions using multiple marker proteins confirmed that the human intrahepatic biliary tree forms through several developmental stages involving an initial transition of primitive hepatocytes into cholangiocytes shaping the ductal plate followed by a process of maturation and remodeling where the intrahepatic biliary tree develops through an asymmetrical form of cholangiocyte tubulogenesis. CONCLUSIONS: The developed protocols provide a novel and sophisticated three-dimensional visualization of vessels and protein expression in human liver during development and disease.

KW - Faculty of Health and Medical Sciences

U2 - 10.1186/1471-213X-11-56

DO - 10.1186/1471-213X-11-56

M3 - Journal article

C2 - 21943389

VL - 11

SP - 56

JO - B M C Developmental Biology

JF - B M C Developmental Biology

SN - 1471-213X

ER -

ID: 34470853