X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase. / Yennawar, Hemant; Møller, Magda; Gillilan, Richard; Yennawar, Neela.

In: Acta Crystallographica. Section D: Biological Crystallography, Vol. 67, No. Pt 5, 05.2011, p. 440-446.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Yennawar, H, Møller, M, Gillilan, R & Yennawar, N 2011, 'X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase', Acta Crystallographica. Section D: Biological Crystallography, vol. 67, no. Pt 5, pp. 440-446. https://doi.org/10.1107/S0907444911007815

APA

Yennawar, H., Møller, M., Gillilan, R., & Yennawar, N. (2011). X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase. Acta Crystallographica. Section D: Biological Crystallography, 67(Pt 5), 440-446. https://doi.org/10.1107/S0907444911007815

Vancouver

Yennawar H, Møller M, Gillilan R, Yennawar N. X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase. Acta Crystallographica. Section D: Biological Crystallography. 2011 May;67(Pt 5):440-446. https://doi.org/10.1107/S0907444911007815

Author

Yennawar, Hemant ; Møller, Magda ; Gillilan, Richard ; Yennawar, Neela. / X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase. In: Acta Crystallographica. Section D: Biological Crystallography. 2011 ; Vol. 67, No. Pt 5. pp. 440-446.

Bibtex

@article{7a85495ab5f54df4952b4fabe3670924,
title = "X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase",
abstract = "The X-ray crystal structure of sheep liver sorbitol dehydrogenase (slSDH) has been determined using the crystal structure of human sorbitol dehydrogenase (hSDH) as a molecular-replacement model. slSDH crystallized in space group I222 with one monomer in the asymmetric unit. A conserved tetramer that superposes well with that seen in hSDH (despite belonging to a different space group) and obeying the 222 crystal symmetry is seen in slSDH. An acetate molecule is bound in the active site, coordinating to the active-site zinc through a water molecule. Glycerol, a substrate of slSDH, also occupies the substrate-binding pocket together with the acetate designed by nature to fit large polyol substrates. The substrate-binding pocket is seen to be in close proximity to the tetramer interface, which explains the need for the structural integrity of the tetramer for enzyme activity. Small-angle X-ray scattering was also used to identify the quaternary structure of the tetramer of slSDH in solution.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Hemant Yennawar and Magda M{\o}ller and Richard Gillilan and Neela Yennawar",
note = "Keywords: sheep liver sorbitol dehydrogenase; 3qe3",
year = "2011",
month = "5",
doi = "10.1107/S0907444911007815",
language = "English",
volume = "67",
pages = "440--446",
journal = "Acta Crystallographica Section D: Structural Biology",
issn = "2059-7983",
publisher = "International Union of Crystallography",
number = "Pt 5",

}

RIS

TY - JOUR

T1 - X-ray crystal structure and small-angle X-ray scattering of sheep liver sorbitol dehydrogenase

AU - Yennawar, Hemant

AU - Møller, Magda

AU - Gillilan, Richard

AU - Yennawar, Neela

N1 - Keywords: sheep liver sorbitol dehydrogenase; 3qe3

PY - 2011/5

Y1 - 2011/5

N2 - The X-ray crystal structure of sheep liver sorbitol dehydrogenase (slSDH) has been determined using the crystal structure of human sorbitol dehydrogenase (hSDH) as a molecular-replacement model. slSDH crystallized in space group I222 with one monomer in the asymmetric unit. A conserved tetramer that superposes well with that seen in hSDH (despite belonging to a different space group) and obeying the 222 crystal symmetry is seen in slSDH. An acetate molecule is bound in the active site, coordinating to the active-site zinc through a water molecule. Glycerol, a substrate of slSDH, also occupies the substrate-binding pocket together with the acetate designed by nature to fit large polyol substrates. The substrate-binding pocket is seen to be in close proximity to the tetramer interface, which explains the need for the structural integrity of the tetramer for enzyme activity. Small-angle X-ray scattering was also used to identify the quaternary structure of the tetramer of slSDH in solution.

AB - The X-ray crystal structure of sheep liver sorbitol dehydrogenase (slSDH) has been determined using the crystal structure of human sorbitol dehydrogenase (hSDH) as a molecular-replacement model. slSDH crystallized in space group I222 with one monomer in the asymmetric unit. A conserved tetramer that superposes well with that seen in hSDH (despite belonging to a different space group) and obeying the 222 crystal symmetry is seen in slSDH. An acetate molecule is bound in the active site, coordinating to the active-site zinc through a water molecule. Glycerol, a substrate of slSDH, also occupies the substrate-binding pocket together with the acetate designed by nature to fit large polyol substrates. The substrate-binding pocket is seen to be in close proximity to the tetramer interface, which explains the need for the structural integrity of the tetramer for enzyme activity. Small-angle X-ray scattering was also used to identify the quaternary structure of the tetramer of slSDH in solution.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1107/S0907444911007815

DO - 10.1107/S0907444911007815

M3 - Journal article

C2 - 21543846

VL - 67

SP - 440

EP - 446

JO - Acta Crystallographica Section D: Structural Biology

JF - Acta Crystallographica Section D: Structural Biology

SN - 2059-7983

IS - Pt 5

ER -

ID: 38145892