Campylobacter concisus: an evaluation of certain phenotypic and genotypic characteristics
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Campylobacter concisus : an evaluation of certain phenotypic and genotypic characteristics. / Engberg, J; Bang, D D; Aabenhus, Rune Munck; Aarestrup, F M; Fussing, V; Gerner-Smidt, P.
In: Clinical Microbiology and Infection, Vol. 11, No. 4, 04.2005, p. 288-95.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Campylobacter concisus
T2 - an evaluation of certain phenotypic and genotypic characteristics
AU - Engberg, J
AU - Bang, D D
AU - Aabenhus, Rune Munck
AU - Aarestrup, F M
AU - Fussing, V
AU - Gerner-Smidt, P
PY - 2005/4
Y1 - 2005/4
N2 - The clinical relevance of Campylobacter concisus in gastrointestinal disease has not been determined definitively. This study investigated the phenotypic and genotypic characteristics of 39 C. concisus isolates from Danish patients with diarrhoea, three isolates from healthy individuals and the type strain. A cytolethal distending toxin (CDT)-like effect on Vero cells was observed in 35 (90%) isolates from patients with diarrhoea, in all three isolates from healthy individuals and in the type strain. Analysis of SDS-PAGE protein profiles and PCR amplification of 23S rDNA assigned the isolates into two distinct, but discordant groups. Automated ribotyping (RiboPrinting) identified 34 distinct patterns among the 43 isolates, but cluster analysis did not separate isolates from patients with diarrhoea from isolates from healthy patients. Random amplified polymorphic DNA (RAPD) analysis with three primers identified 37 unique profiles, but requires further evaluation. The isolates obtained from healthy carriers were distinguished by cluster analysis from the isolates obtained from patients with diarrhoea. All the isolates were susceptible to 11 antimicrobial agents tested. Overall, there was considerable variability between the C. concisus isolates, but there were no clear phenotypic or genotypic differences between isolates from patients with diarrhoea and isolates from healthy carriers. Further evidence is needed to support the possible role of C. concisus as a human enteric pathogen.
AB - The clinical relevance of Campylobacter concisus in gastrointestinal disease has not been determined definitively. This study investigated the phenotypic and genotypic characteristics of 39 C. concisus isolates from Danish patients with diarrhoea, three isolates from healthy individuals and the type strain. A cytolethal distending toxin (CDT)-like effect on Vero cells was observed in 35 (90%) isolates from patients with diarrhoea, in all three isolates from healthy individuals and in the type strain. Analysis of SDS-PAGE protein profiles and PCR amplification of 23S rDNA assigned the isolates into two distinct, but discordant groups. Automated ribotyping (RiboPrinting) identified 34 distinct patterns among the 43 isolates, but cluster analysis did not separate isolates from patients with diarrhoea from isolates from healthy patients. Random amplified polymorphic DNA (RAPD) analysis with three primers identified 37 unique profiles, but requires further evaluation. The isolates obtained from healthy carriers were distinguished by cluster analysis from the isolates obtained from patients with diarrhoea. All the isolates were susceptible to 11 antimicrobial agents tested. Overall, there was considerable variability between the C. concisus isolates, but there were no clear phenotypic or genotypic differences between isolates from patients with diarrhoea and isolates from healthy carriers. Further evidence is needed to support the possible role of C. concisus as a human enteric pathogen.
KW - Animals
KW - Bacterial Toxins
KW - Campylobacter
KW - Campylobacter Infections
KW - Carrier State
KW - Cercopithecus aethiops
KW - Cytotoxins
KW - Denmark
KW - Diarrhea
KW - Humans
KW - Phenotype
KW - RNA, Ribosomal, 23S
KW - Random Amplified Polymorphic DNA Technique
KW - Ribotyping
KW - Vero Cells
U2 - 10.1111/j.1469-0691.2005.01111.x
DO - 10.1111/j.1469-0691.2005.01111.x
M3 - Journal article
C2 - 15760425
VL - 11
SP - 288
EP - 295
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
SN - 1198-743X
IS - 4
ER -
ID: 45439847