Bioavailability of seocalcitol IV: evaluation of lymphatic transport in conscious rats

Research output: Contribution to journalJournal articleResearchpeer-review

Mette Grove, Jeanet L Nielsen, Gitte P Pedersen, Anette Müllertz

PURPOSE: To study the use of long chain triglycerides (LCT) as a lymphotropic carrier of (3)H-seocalcitol by comparing the lymphatic transport and the portal absorption of (3)H-seocalcitol when dissolved in a (1) LCT solution or a (2) reference solution without lipid containing propylene glycol (PG). MATERIALS AND METHODS: A lymph cannulated conscious rat model was dosed orally with (3)H-seocalcitol dissolved in either LCT or PG. Lymph was collected continuously, and blood was sampled over 9 h. (3)H-seocalcitol in blood and lymph and triglycerides in lymph were analysed. RESULTS: A statistically significantly (p < 0.05) higher recovery of the dosed (3)H-seocalcitol was found in the intestinal lymph upon administration of the LCT solution (1.3 +/- 0.6%) compared to the PG solution (0.5 +/- 0.4%). The portal absorption of (3)H-seocalcitol was significantly (p < 0.05) higher from the LCT solution (16.2 +/- 2.2%) than from the PG solution (10.8 +/- 0.8%). CONCLUSIONS: The LCT solution resulted in a statistical significantly higher level of lymphatic and portal transport of (3)H-seocalcitol compared with the PG solution. However, even though LCT facilitates the formation of chylomicrons, (3)H-seocalcitol favours absorption directly to the portal blood probably due to the moderate lipophilicity of the molecule.
Original languageEnglish
JournalPharmaceutical Research
Volume23
Issue number11
Pages (from-to)2681-8
Number of pages7
ISSN0724-8741
DOIs
Publication statusPublished - 2006

Bibliographical note

Keywords: Animals; Biological Availability; Biological Transport; Calcitriol; Gastrointestinal Tract; Lymph; Rats; Triglycerides

ID: 9010043