Comparison of techniques for quantification of next-generation sequencing libraries

Research output: Contribution to journalConference articlepeer-review

To ensure efficient sequencing, the DNA of next-generation sequencing (NGS) libraries must be quantified correctly. Therefore, an accurate, sensitive and stable method for DNA quantification is crucial. In this study, seven different methods for DNA quantification were compared to each other by quantifying NGS
libraries for the Ion TorrentTM and Illumina1 platforms as well as dsDNA oligos with known DNA concentrations. Rather large variations in library concentration estimates were observed. The differences between the highest and lowest concentration estimates varied with a factor of 5–100 depending on the
library concentration. The Bioanalyzer, TapeStation and Qubit1 instruments gave concentrations closest to the expected when quantifying dsDNA oligos. At very low concentrations (2–4 pg/ul) only the Bioanalyzer could reliably quantify the dsDNA oligos.
Original languageEnglish
JournalForensic Science International: Genetics. Supplement Series
Volume5
Pages (from-to)e276-e278
Number of pages3
ISSN1875-1768
DOIs
Publication statusPublished - Dec 2015
Event26th Congress of the International Society of Forensic Genetics - Krakow, Poland
Duration: 31 Aug 20155 Sep 2015

Conference

Conference26th Congress of the International Society of Forensic Genetics
CountryPoland
CityKrakow
Period31/08/201505/09/2015

ID: 152269631