High-throughput verification of transcriptional starting sites by Deep-RACE
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- BTN A 000113066 O 12907a
Final published version, 465 KB, PDF document
We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.
| Original language | English |
|---|---|
| Journal | BioTechniques |
| Volume | 46 |
| Issue number | 2 |
| Pages (from-to) | 130-132 |
| Number of pages | 3 |
| ISSN | 0736-6205 |
| DOIs | |
| Publication status | Published - 2009 |
- Faculty of Science - promoters, transcription start sites, high throughput, RACE, short-reads sequencing
Research areas
Number of downloads are based on statistics from Google Scholar and www.ku.dk
No data available
ID: 17880276