Human platelet lysate improves the growth and survival of cultured human pre-antral follicles
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Human platelet lysate improves the growth and survival of cultured human pre-antral follicles. / Subiran Adrados, Cristina; Cadenas, Jesús; Zheng, Mengxue; Lund, Sofie; Larsen, Elisabeth Clare; Tanvig, Mette Honnens; Greve, Vinnie Hornshøj; Blanche, Paul; Andersen, Claus Yding; Kristensen, Stine Gry.
In: Reproductive BioMedicine Online, Vol. 47, No. 5, 103256, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Human platelet lysate improves the growth and survival of cultured human pre-antral follicles
AU - Subiran Adrados, Cristina
AU - Cadenas, Jesús
AU - Zheng, Mengxue
AU - Lund, Sofie
AU - Larsen, Elisabeth Clare
AU - Tanvig, Mette Honnens
AU - Greve, Vinnie Hornshøj
AU - Blanche, Paul
AU - Andersen, Claus Yding
AU - Kristensen, Stine Gry
N1 - Publisher Copyright: © 2023 The Author(s)
PY - 2023
Y1 - 2023
N2 - Research question: How do platelet-rich plasma products like human platelet lysate (HPL) and umbilical cord plasma (UCP) affect the growth and survival of isolated human pre-antral follicles in vitro? Design: Human pre-antral follicles (n = 724; mean diameter: 75 µm; range: 46–237 µm) were isolated from ovarian medulla donated by 14 patients undergoing unilateral oophorectomy for ovarian tissue cryopreservation. Follicles were encapsulated in 0.5% alginate and cultured for 8 days in media supplemented with 5% fetal bovine serum (FBS) (n = 171), 2.5% human serum albumin (HSA) (n = 159), 5% HPL (n = 223) or 5% UCP (n = 171). Results: The survival probability was significantly higher in the group supplemented with HPL (80%) compared with the other three groups: FBS (54%, P < 0.001); HSA (63%, P = 0.004) and UCP (29%, P < 0.001). Surviving follicles in the UCP group had less defined follicular membranes and decompacted granulosa cell layers. The median growth of surviving follicles was significantly (P < 0.001) larger in the HPL group (73 µm) compared with any of the other three groups: HSA (43 μm); FBS (40 μm) UCP (54 μm). A descriptive analysis of follicular secretion of anti-Müllerian hormone and oestradiol did not reveal any difference between the groups. The detectability of follicular genes was high for AR (100%), AMHR2 (100%) and FSHR (76%), whereas few follicles expressed LHR (20%). Conclusion: Human platelet lysate significantly improved survival and growth of cultured human pre-antral follicles compared with FBS, HSA and UCP. The use of HPL is a valuable improvement to culture human pre-antral follicles but further studies will have to prove whether the superiority of HPL translates into better quality oocytes.
AB - Research question: How do platelet-rich plasma products like human platelet lysate (HPL) and umbilical cord plasma (UCP) affect the growth and survival of isolated human pre-antral follicles in vitro? Design: Human pre-antral follicles (n = 724; mean diameter: 75 µm; range: 46–237 µm) were isolated from ovarian medulla donated by 14 patients undergoing unilateral oophorectomy for ovarian tissue cryopreservation. Follicles were encapsulated in 0.5% alginate and cultured for 8 days in media supplemented with 5% fetal bovine serum (FBS) (n = 171), 2.5% human serum albumin (HSA) (n = 159), 5% HPL (n = 223) or 5% UCP (n = 171). Results: The survival probability was significantly higher in the group supplemented with HPL (80%) compared with the other three groups: FBS (54%, P < 0.001); HSA (63%, P = 0.004) and UCP (29%, P < 0.001). Surviving follicles in the UCP group had less defined follicular membranes and decompacted granulosa cell layers. The median growth of surviving follicles was significantly (P < 0.001) larger in the HPL group (73 µm) compared with any of the other three groups: HSA (43 μm); FBS (40 μm) UCP (54 μm). A descriptive analysis of follicular secretion of anti-Müllerian hormone and oestradiol did not reveal any difference between the groups. The detectability of follicular genes was high for AR (100%), AMHR2 (100%) and FSHR (76%), whereas few follicles expressed LHR (20%). Conclusion: Human platelet lysate significantly improved survival and growth of cultured human pre-antral follicles compared with FBS, HSA and UCP. The use of HPL is a valuable improvement to culture human pre-antral follicles but further studies will have to prove whether the superiority of HPL translates into better quality oocytes.
KW - fertility preservation
KW - follicle culture
KW - human pre-antral follicles
KW - platelet rich plasma
U2 - 10.1016/j.rbmo.2023.06.011
DO - 10.1016/j.rbmo.2023.06.011
M3 - Journal article
C2 - 37690342
AN - SCOPUS:85167891497
VL - 47
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
SN - 1472-6483
IS - 5
M1 - 103256
ER -
ID: 371126040