In vivo toxicity of cationic micelles and liposomes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

In vivo toxicity of cationic micelles and liposomes. / Knudsen, Kristina Bram; Northeved, Helle; Kumar Ek, Pramod; Permin, Anders; Gjetting, Torben; Andresen, Thomas L; Larsen, Steen; Wegener, Karen Malene; Lykkesfeldt, Jens; Jantzen, Kim; Loft, Steffen; Møller, Peter; Roursgaard, Martin.

In: Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 11, No. 2, 25.02.2015, p. 467-477.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Knudsen, KB, Northeved, H, Kumar Ek, P, Permin, A, Gjetting, T, Andresen, TL, Larsen, S, Wegener, KM, Lykkesfeldt, J, Jantzen, K, Loft, S, Møller, P & Roursgaard, M 2015, 'In vivo toxicity of cationic micelles and liposomes', Nanomedicine: Nanotechnology, Biology and Medicine, vol. 11, no. 2, pp. 467-477. https://doi.org/10.1016/j.nano.2014.08.004

APA

Knudsen, K. B., Northeved, H., Kumar Ek, P., Permin, A., Gjetting, T., Andresen, T. L., Larsen, S., Wegener, K. M., Lykkesfeldt, J., Jantzen, K., Loft, S., Møller, P., & Roursgaard, M. (2015). In vivo toxicity of cationic micelles and liposomes. Nanomedicine: Nanotechnology, Biology and Medicine, 11(2), 467-477. https://doi.org/10.1016/j.nano.2014.08.004

Vancouver

Knudsen KB, Northeved H, Kumar Ek P, Permin A, Gjetting T, Andresen TL et al. In vivo toxicity of cationic micelles and liposomes. Nanomedicine: Nanotechnology, Biology and Medicine. 2015 Feb 25;11(2):467-477. https://doi.org/10.1016/j.nano.2014.08.004

Author

Knudsen, Kristina Bram ; Northeved, Helle ; Kumar Ek, Pramod ; Permin, Anders ; Gjetting, Torben ; Andresen, Thomas L ; Larsen, Steen ; Wegener, Karen Malene ; Lykkesfeldt, Jens ; Jantzen, Kim ; Loft, Steffen ; Møller, Peter ; Roursgaard, Martin. / In vivo toxicity of cationic micelles and liposomes. In: Nanomedicine: Nanotechnology, Biology and Medicine. 2015 ; Vol. 11, No. 2. pp. 467-477.

Bibtex

@article{c5f3d2b1fb094e9fbaa64dffc1228ab0,
title = "In vivo toxicity of cationic micelles and liposomes",
abstract = "This study investigated toxicity of nanocarriers comprised of cationic polymer and lipid components often used in gene and drug delivery, formulated as cationic micelles and liposomes. Rats were injected intravenously with 10, 25 or 100mg/kg and sacrificed after 24 or 48h, or 24h after the last of three intravenous injections of 100mg/kg every other day. Histological evaluation of liver, lung and spleen, clinical chemistry parameters, and hematology indicated little effect of treatment. DNA strand breaks were increased in the lung and spleen. Further, in the dose response study we found unaltered expression levels of genes in the antioxidant response (HMOX1) and repair of oxidized nucleobases (OGG1), whereas expression levels of cytokines (IL6, CXCL2 and CCL2) were elevated in lung, spleen or liver. The results indicate that assessment of genotoxicity and gene expression add information on toxicity of nanocarriers, which is not obtained by histology and hematology.",
keywords = "Faculty of Health and Medical Sciences, Nanocarriers, Liposomes, Micelles, In vivo toxicology, Nanomedicine",
author = "Knudsen, {Kristina Bram} and Helle Northeved and {Kumar Ek}, Pramod and Anders Permin and Torben Gjetting and Andresen, {Thomas L} and Steen Larsen and Wegener, {Karen Malene} and Jens Lykkesfeldt and Kim Jantzen and Steffen Loft and Peter M{\o}ller and Martin Roursgaard",
note = "Copyright {\textcopyright} 2014. Published by Elsevier Inc.",
year = "2015",
month = feb,
day = "25",
doi = "10.1016/j.nano.2014.08.004",
language = "English",
volume = "11",
pages = "467--477",
journal = "Nanomedicine: Nanotechnology, Biology and Medicine",
issn = "1549-9634",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - In vivo toxicity of cationic micelles and liposomes

AU - Knudsen, Kristina Bram

AU - Northeved, Helle

AU - Kumar Ek, Pramod

AU - Permin, Anders

AU - Gjetting, Torben

AU - Andresen, Thomas L

AU - Larsen, Steen

AU - Wegener, Karen Malene

AU - Lykkesfeldt, Jens

AU - Jantzen, Kim

AU - Loft, Steffen

AU - Møller, Peter

AU - Roursgaard, Martin

N1 - Copyright © 2014. Published by Elsevier Inc.

PY - 2015/2/25

Y1 - 2015/2/25

N2 - This study investigated toxicity of nanocarriers comprised of cationic polymer and lipid components often used in gene and drug delivery, formulated as cationic micelles and liposomes. Rats were injected intravenously with 10, 25 or 100mg/kg and sacrificed after 24 or 48h, or 24h after the last of three intravenous injections of 100mg/kg every other day. Histological evaluation of liver, lung and spleen, clinical chemistry parameters, and hematology indicated little effect of treatment. DNA strand breaks were increased in the lung and spleen. Further, in the dose response study we found unaltered expression levels of genes in the antioxidant response (HMOX1) and repair of oxidized nucleobases (OGG1), whereas expression levels of cytokines (IL6, CXCL2 and CCL2) were elevated in lung, spleen or liver. The results indicate that assessment of genotoxicity and gene expression add information on toxicity of nanocarriers, which is not obtained by histology and hematology.

AB - This study investigated toxicity of nanocarriers comprised of cationic polymer and lipid components often used in gene and drug delivery, formulated as cationic micelles and liposomes. Rats were injected intravenously with 10, 25 or 100mg/kg and sacrificed after 24 or 48h, or 24h after the last of three intravenous injections of 100mg/kg every other day. Histological evaluation of liver, lung and spleen, clinical chemistry parameters, and hematology indicated little effect of treatment. DNA strand breaks were increased in the lung and spleen. Further, in the dose response study we found unaltered expression levels of genes in the antioxidant response (HMOX1) and repair of oxidized nucleobases (OGG1), whereas expression levels of cytokines (IL6, CXCL2 and CCL2) were elevated in lung, spleen or liver. The results indicate that assessment of genotoxicity and gene expression add information on toxicity of nanocarriers, which is not obtained by histology and hematology.

KW - Faculty of Health and Medical Sciences

KW - Nanocarriers

KW - Liposomes

KW - Micelles

KW - In vivo toxicology

KW - Nanomedicine

U2 - 10.1016/j.nano.2014.08.004

DO - 10.1016/j.nano.2014.08.004

M3 - Journal article

C2 - 25168934

VL - 11

SP - 467

EP - 477

JO - Nanomedicine: Nanotechnology, Biology and Medicine

JF - Nanomedicine: Nanotechnology, Biology and Medicine

SN - 1549-9634

IS - 2

ER -

ID: 124426961