Measuring DNA modifications with the comet assay: a compendium of protocols

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Measuring DNA modifications with the comet assay : a compendium of protocols. / Collins, Andrew; Moller, Peter; Gajski, Goran; Vodenkova, Sona; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, Stefano; Boutet-Robinet, Elisa; Brunborg, Gunnar; Chao, Christy; Cooke, Marcus S. S.; Costa, Carla; Costa, Solange; Dhawan, Alok; de Lapuente, Joaquin; Del Bo, Cristian; Dubus, Julien; Dusinska, Maria; Duthie, Susan J. J.; El Yamani, Naouale; Engelward, Bevin; Gaivao, Isabel; Giovannelli, Lisa; Godschalk, Roger; Guilherme, Sofia; Gutzkow, Kristine B. B.; Habas, Khaled; Hernandez, Alba; Herrero, Oscar; Isidori, Marina; Jha, Awadhesh N. N.; Knasmueller, Siegfried; Kooter, Ingeborg M. M.; Koppen, Gudrun; Kruszewski, Marcin; Ladeira, Carina; Laffon, Blanca; Larramendy, Marcelo; Le Hegarat, Ludovic; Lewies, Angelique; Lewinska, Anna; Liwszyc, Guillermo E. E.; de Cerain, Adela Lopez; Manjanatha, Mugimane; Marcos, Ricard; Milic, Mirta; de Andrade, Vanessa Moraes; Moretti, Massimo; Muruzabal, Damian; Novak, Matjaz; Oliveira, Rui; Olsen, Ann-Karin; Owiti, Norah; Pacheco, Mario; Pandey, Alok K. K.; Pfuhler, Stefan; Pourrut, Bertrand; Reisinger, Kerstin; Rojas, Emilio; Runden-Pran, Elise; Sanz-Serrano, Julen; Shaposhnikov, Sergey; Sipinen, Ville; Smeets, Karen; Stopper, Helga; Teixeira, Joao Paulo; Valdiglesias, Vanessa; Valverde, Mahara; van Acker, Frederique; van Schooten, Frederik-Jan; Vasquez, Marie; Wentzel, Johannes F. F.; Wnuk, Maciej; Wouters, Annelies; Zegura, Bojana; Zikmund, Tomas; Langie, Sabine A. S.; Azqueta, Amaya.

In: Nature Protocols, Vol. 18, 2023, p. 929–989.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Collins, A, Moller, P, Gajski, G, Vodenkova, S, Abdulwahed, A, Anderson, D, Bankoglu, EE, Bonassi, S, Boutet-Robinet, E, Brunborg, G, Chao, C, Cooke, MSS, Costa, C, Costa, S, Dhawan, A, de Lapuente, J, Del Bo, C, Dubus, J, Dusinska, M, Duthie, SJJ, El Yamani, N, Engelward, B, Gaivao, I, Giovannelli, L, Godschalk, R, Guilherme, S, Gutzkow, KBB, Habas, K, Hernandez, A, Herrero, O, Isidori, M, Jha, ANN, Knasmueller, S, Kooter, IMM, Koppen, G, Kruszewski, M, Ladeira, C, Laffon, B, Larramendy, M, Le Hegarat, L, Lewies, A, Lewinska, A, Liwszyc, GEE, de Cerain, AL, Manjanatha, M, Marcos, R, Milic, M, de Andrade, VM, Moretti, M, Muruzabal, D, Novak, M, Oliveira, R, Olsen, A-K, Owiti, N, Pacheco, M, Pandey, AKK, Pfuhler, S, Pourrut, B, Reisinger, K, Rojas, E, Runden-Pran, E, Sanz-Serrano, J, Shaposhnikov, S, Sipinen, V, Smeets, K, Stopper, H, Teixeira, JP, Valdiglesias, V, Valverde, M, van Acker, F, van Schooten, F-J, Vasquez, M, Wentzel, JFF, Wnuk, M, Wouters, A, Zegura, B, Zikmund, T, Langie, SAS & Azqueta, A 2023, 'Measuring DNA modifications with the comet assay: a compendium of protocols', Nature Protocols, vol. 18, pp. 929–989. https://doi.org/10.1038/s41596-022-00754-y

APA

Collins, A., Moller, P., Gajski, G., Vodenkova, S., Abdulwahed, A., Anderson, D., Bankoglu, E. E., Bonassi, S., Boutet-Robinet, E., Brunborg, G., Chao, C., Cooke, M. S. S., Costa, C., Costa, S., Dhawan, A., de Lapuente, J., Del Bo, C., Dubus, J., Dusinska, M., ... Azqueta, A. (2023). Measuring DNA modifications with the comet assay: a compendium of protocols. Nature Protocols, 18, 929–989. https://doi.org/10.1038/s41596-022-00754-y

Vancouver

Collins A, Moller P, Gajski G, Vodenkova S, Abdulwahed A, Anderson D et al. Measuring DNA modifications with the comet assay: a compendium of protocols. Nature Protocols. 2023;18:929–989. https://doi.org/10.1038/s41596-022-00754-y

Author

Collins, Andrew ; Moller, Peter ; Gajski, Goran ; Vodenkova, Sona ; Abdulwahed, Abdulhadi ; Anderson, Diana ; Bankoglu, Ezgi Eyluel ; Bonassi, Stefano ; Boutet-Robinet, Elisa ; Brunborg, Gunnar ; Chao, Christy ; Cooke, Marcus S. S. ; Costa, Carla ; Costa, Solange ; Dhawan, Alok ; de Lapuente, Joaquin ; Del Bo, Cristian ; Dubus, Julien ; Dusinska, Maria ; Duthie, Susan J. J. ; El Yamani, Naouale ; Engelward, Bevin ; Gaivao, Isabel ; Giovannelli, Lisa ; Godschalk, Roger ; Guilherme, Sofia ; Gutzkow, Kristine B. B. ; Habas, Khaled ; Hernandez, Alba ; Herrero, Oscar ; Isidori, Marina ; Jha, Awadhesh N. N. ; Knasmueller, Siegfried ; Kooter, Ingeborg M. M. ; Koppen, Gudrun ; Kruszewski, Marcin ; Ladeira, Carina ; Laffon, Blanca ; Larramendy, Marcelo ; Le Hegarat, Ludovic ; Lewies, Angelique ; Lewinska, Anna ; Liwszyc, Guillermo E. E. ; de Cerain, Adela Lopez ; Manjanatha, Mugimane ; Marcos, Ricard ; Milic, Mirta ; de Andrade, Vanessa Moraes ; Moretti, Massimo ; Muruzabal, Damian ; Novak, Matjaz ; Oliveira, Rui ; Olsen, Ann-Karin ; Owiti, Norah ; Pacheco, Mario ; Pandey, Alok K. K. ; Pfuhler, Stefan ; Pourrut, Bertrand ; Reisinger, Kerstin ; Rojas, Emilio ; Runden-Pran, Elise ; Sanz-Serrano, Julen ; Shaposhnikov, Sergey ; Sipinen, Ville ; Smeets, Karen ; Stopper, Helga ; Teixeira, Joao Paulo ; Valdiglesias, Vanessa ; Valverde, Mahara ; van Acker, Frederique ; van Schooten, Frederik-Jan ; Vasquez, Marie ; Wentzel, Johannes F. F. ; Wnuk, Maciej ; Wouters, Annelies ; Zegura, Bojana ; Zikmund, Tomas ; Langie, Sabine A. S. ; Azqueta, Amaya. / Measuring DNA modifications with the comet assay : a compendium of protocols. In: Nature Protocols. 2023 ; Vol. 18. pp. 929–989.

Bibtex

@article{a047e9bb1d914c7fba346f6e27c1d89b,
title = "Measuring DNA modifications with the comet assay: a compendium of protocols",
abstract = "The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.",
keywords = "CELL GEL-ELECTROPHORESIS, DOUBLE-STRAND BREAKS, IN-VIVO MODEL, INTER-LABORATORY VARIATION, INDUCED OXIDATIVE STRESS, INTERSTRAND CROSS-LINKS, ENABLES HIGH-THROUGHPUT, DROSOPHILA-MELANOGASTER, EPITHELIAL-CELLS, CORD BLOOD",
author = "Andrew Collins and Peter Moller and Goran Gajski and Sona Vodenkova and Abdulhadi Abdulwahed and Diana Anderson and Bankoglu, {Ezgi Eyluel} and Stefano Bonassi and Elisa Boutet-Robinet and Gunnar Brunborg and Christy Chao and Cooke, {Marcus S. S.} and Carla Costa and Solange Costa and Alok Dhawan and {de Lapuente}, Joaquin and {Del Bo}, Cristian and Julien Dubus and Maria Dusinska and Duthie, {Susan J. J.} and {El Yamani}, Naouale and Bevin Engelward and Isabel Gaivao and Lisa Giovannelli and Roger Godschalk and Sofia Guilherme and Gutzkow, {Kristine B. B.} and Khaled Habas and Alba Hernandez and Oscar Herrero and Marina Isidori and Jha, {Awadhesh N. N.} and Siegfried Knasmueller and Kooter, {Ingeborg M. M.} and Gudrun Koppen and Marcin Kruszewski and Carina Ladeira and Blanca Laffon and Marcelo Larramendy and {Le Hegarat}, Ludovic and Angelique Lewies and Anna Lewinska and Liwszyc, {Guillermo E. E.} and {de Cerain}, {Adela Lopez} and Mugimane Manjanatha and Ricard Marcos and Mirta Milic and {de Andrade}, {Vanessa Moraes} and Massimo Moretti and Damian Muruzabal and Matjaz Novak and Rui Oliveira and Ann-Karin Olsen and Norah Owiti and Mario Pacheco and Pandey, {Alok K. K.} and Stefan Pfuhler and Bertrand Pourrut and Kerstin Reisinger and Emilio Rojas and Elise Runden-Pran and Julen Sanz-Serrano and Sergey Shaposhnikov and Ville Sipinen and Karen Smeets and Helga Stopper and Teixeira, {Joao Paulo} and Vanessa Valdiglesias and Mahara Valverde and {van Acker}, Frederique and {van Schooten}, Frederik-Jan and Marie Vasquez and Wentzel, {Johannes F. F.} and Maciej Wnuk and Annelies Wouters and Bojana Zegura and Tomas Zikmund and Langie, {Sabine A. S.} and Amaya Azqueta",
year = "2023",
doi = "10.1038/s41596-022-00754-y",
language = "English",
volume = "18",
pages = "929–989",
journal = "Nature Protocols (Online)",
issn = "1750-2799",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Measuring DNA modifications with the comet assay

T2 - a compendium of protocols

AU - Collins, Andrew

AU - Moller, Peter

AU - Gajski, Goran

AU - Vodenkova, Sona

AU - Abdulwahed, Abdulhadi

AU - Anderson, Diana

AU - Bankoglu, Ezgi Eyluel

AU - Bonassi, Stefano

AU - Boutet-Robinet, Elisa

AU - Brunborg, Gunnar

AU - Chao, Christy

AU - Cooke, Marcus S. S.

AU - Costa, Carla

AU - Costa, Solange

AU - Dhawan, Alok

AU - de Lapuente, Joaquin

AU - Del Bo, Cristian

AU - Dubus, Julien

AU - Dusinska, Maria

AU - Duthie, Susan J. J.

AU - El Yamani, Naouale

AU - Engelward, Bevin

AU - Gaivao, Isabel

AU - Giovannelli, Lisa

AU - Godschalk, Roger

AU - Guilherme, Sofia

AU - Gutzkow, Kristine B. B.

AU - Habas, Khaled

AU - Hernandez, Alba

AU - Herrero, Oscar

AU - Isidori, Marina

AU - Jha, Awadhesh N. N.

AU - Knasmueller, Siegfried

AU - Kooter, Ingeborg M. M.

AU - Koppen, Gudrun

AU - Kruszewski, Marcin

AU - Ladeira, Carina

AU - Laffon, Blanca

AU - Larramendy, Marcelo

AU - Le Hegarat, Ludovic

AU - Lewies, Angelique

AU - Lewinska, Anna

AU - Liwszyc, Guillermo E. E.

AU - de Cerain, Adela Lopez

AU - Manjanatha, Mugimane

AU - Marcos, Ricard

AU - Milic, Mirta

AU - de Andrade, Vanessa Moraes

AU - Moretti, Massimo

AU - Muruzabal, Damian

AU - Novak, Matjaz

AU - Oliveira, Rui

AU - Olsen, Ann-Karin

AU - Owiti, Norah

AU - Pacheco, Mario

AU - Pandey, Alok K. K.

AU - Pfuhler, Stefan

AU - Pourrut, Bertrand

AU - Reisinger, Kerstin

AU - Rojas, Emilio

AU - Runden-Pran, Elise

AU - Sanz-Serrano, Julen

AU - Shaposhnikov, Sergey

AU - Sipinen, Ville

AU - Smeets, Karen

AU - Stopper, Helga

AU - Teixeira, Joao Paulo

AU - Valdiglesias, Vanessa

AU - Valverde, Mahara

AU - van Acker, Frederique

AU - van Schooten, Frederik-Jan

AU - Vasquez, Marie

AU - Wentzel, Johannes F. F.

AU - Wnuk, Maciej

AU - Wouters, Annelies

AU - Zegura, Bojana

AU - Zikmund, Tomas

AU - Langie, Sabine A. S.

AU - Azqueta, Amaya

PY - 2023

Y1 - 2023

N2 - The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.

AB - The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.

KW - CELL GEL-ELECTROPHORESIS

KW - DOUBLE-STRAND BREAKS

KW - IN-VIVO MODEL

KW - INTER-LABORATORY VARIATION

KW - INDUCED OXIDATIVE STRESS

KW - INTERSTRAND CROSS-LINKS

KW - ENABLES HIGH-THROUGHPUT

KW - DROSOPHILA-MELANOGASTER

KW - EPITHELIAL-CELLS

KW - CORD BLOOD

U2 - 10.1038/s41596-022-00754-y

DO - 10.1038/s41596-022-00754-y

M3 - Journal article

C2 - 36707722

VL - 18

SP - 929

EP - 989

JO - Nature Protocols (Online)

JF - Nature Protocols (Online)

SN - 1750-2799

ER -

ID: 337783267