Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses

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Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses. / Schoster, Angelika; Arroyo, Luis Guillermo; Staempfli, Henry Rolf; Shewen, Patricia Elisabeth; Weese, Jeffrey Scott.

In: B M C Veterinary Research, Vol. 8, No. 94, 2012.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Schoster, A, Arroyo, LG, Staempfli, HR, Shewen, PE & Weese, JS 2012, 'Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses', B M C Veterinary Research, vol. 8, no. 94. https://doi.org/10.1186/1746-6148-8-94

APA

Schoster, A., Arroyo, L. G., Staempfli, H. R., Shewen, P. E., & Weese, J. S. (2012). Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses. B M C Veterinary Research, 8(94). https://doi.org/10.1186/1746-6148-8-94

Vancouver

Schoster A, Arroyo LG, Staempfli HR, Shewen PE, Weese JS. Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses. B M C Veterinary Research. 2012;8(94). https://doi.org/10.1186/1746-6148-8-94

Author

Schoster, Angelika ; Arroyo, Luis Guillermo ; Staempfli, Henry Rolf ; Shewen, Patricia Elisabeth ; Weese, Jeffrey Scott. / Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses. In: B M C Veterinary Research. 2012 ; Vol. 8, No. 94.

Bibtex

@article{90fe36ac86354f16915976c8bbb6de14,
title = "Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses",
abstract = "BACKGROUND:Clostridium difficile and Clostridium perfringens are commonly associated with colitis in equids, but healthy carriers exist. Scarce information is available on the prevalence of Clostridium spp. in gastrointestinal compartments other than faeces in healthy horses, and it is unknown whether faecal samples are representative of proximal compartments. The objectives were to investigate the prevalence of C. difficile and C. perfringens in different intestinal compartments of healthy adult horses and to determine whether faecal samples are representative of colonization in proximal sites and overall carrier status.RESULTS:Toxigenic C. difficile was isolated from 14/135 (10.3{\%}) samples from 8/15 (53.3{\%}) horses. Between zero and three sites were positive per horse, and multiple sites were positive in four horses. Isolates were recovered from duodenum, jejunum, ileum, right dorsal colon, small colon and rectum. When multiple compartments were positive in a single horse, two different C. difficile ribotypes were always present. Clostridium perfringens Type A (CPE, beta2 toxin gene negative) was recovered from the left ventral colon of one horse (0.74{\%}, 1/135 samples). Agreement between faeces and overall C. difficile carrier status was good.CONCLUSIONS:Clostridium difficile can be found in different compartments of the gastrointestinal tract of healthy horses, and multiple strains can be present in an individual horse. The prevalence of C. perfringens in healthy adult hoses was low, consistent with previous reports. Faecal samples were representative for presence of C. difficile in proximal compartments in 5/8 horses (63{\%}) but were not representative for the specific strain.",
keywords = "Faculty of Health and Medical Sciences, Clostridium difficile, Clostridium perfringens, Equine gastrointestinal tract, Equine intestinal microflora, Clostridium difficile ribotyping, Former LIFE faculty, Clostridium difficile, Clostridium perfringens, equine gastrointestinal tract, equine intestinal microflora, Clostridium difficile ribotyping",
author = "Angelika Schoster and Arroyo, {Luis Guillermo} and Staempfli, {Henry Rolf} and Shewen, {Patricia Elisabeth} and Weese, {Jeffrey Scott}",
year = "2012",
doi = "10.1186/1746-6148-8-94",
language = "English",
volume = "8",
journal = "B M C Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central Ltd.",
number = "94",

}

RIS

TY - JOUR

T1 - Presence and molecular characterization of Clostridium difficile and Clostridium perfringens in intestinal compartments of healthy horses

AU - Schoster, Angelika

AU - Arroyo, Luis Guillermo

AU - Staempfli, Henry Rolf

AU - Shewen, Patricia Elisabeth

AU - Weese, Jeffrey Scott

PY - 2012

Y1 - 2012

N2 - BACKGROUND:Clostridium difficile and Clostridium perfringens are commonly associated with colitis in equids, but healthy carriers exist. Scarce information is available on the prevalence of Clostridium spp. in gastrointestinal compartments other than faeces in healthy horses, and it is unknown whether faecal samples are representative of proximal compartments. The objectives were to investigate the prevalence of C. difficile and C. perfringens in different intestinal compartments of healthy adult horses and to determine whether faecal samples are representative of colonization in proximal sites and overall carrier status.RESULTS:Toxigenic C. difficile was isolated from 14/135 (10.3%) samples from 8/15 (53.3%) horses. Between zero and three sites were positive per horse, and multiple sites were positive in four horses. Isolates were recovered from duodenum, jejunum, ileum, right dorsal colon, small colon and rectum. When multiple compartments were positive in a single horse, two different C. difficile ribotypes were always present. Clostridium perfringens Type A (CPE, beta2 toxin gene negative) was recovered from the left ventral colon of one horse (0.74%, 1/135 samples). Agreement between faeces and overall C. difficile carrier status was good.CONCLUSIONS:Clostridium difficile can be found in different compartments of the gastrointestinal tract of healthy horses, and multiple strains can be present in an individual horse. The prevalence of C. perfringens in healthy adult hoses was low, consistent with previous reports. Faecal samples were representative for presence of C. difficile in proximal compartments in 5/8 horses (63%) but were not representative for the specific strain.

AB - BACKGROUND:Clostridium difficile and Clostridium perfringens are commonly associated with colitis in equids, but healthy carriers exist. Scarce information is available on the prevalence of Clostridium spp. in gastrointestinal compartments other than faeces in healthy horses, and it is unknown whether faecal samples are representative of proximal compartments. The objectives were to investigate the prevalence of C. difficile and C. perfringens in different intestinal compartments of healthy adult horses and to determine whether faecal samples are representative of colonization in proximal sites and overall carrier status.RESULTS:Toxigenic C. difficile was isolated from 14/135 (10.3%) samples from 8/15 (53.3%) horses. Between zero and three sites were positive per horse, and multiple sites were positive in four horses. Isolates were recovered from duodenum, jejunum, ileum, right dorsal colon, small colon and rectum. When multiple compartments were positive in a single horse, two different C. difficile ribotypes were always present. Clostridium perfringens Type A (CPE, beta2 toxin gene negative) was recovered from the left ventral colon of one horse (0.74%, 1/135 samples). Agreement between faeces and overall C. difficile carrier status was good.CONCLUSIONS:Clostridium difficile can be found in different compartments of the gastrointestinal tract of healthy horses, and multiple strains can be present in an individual horse. The prevalence of C. perfringens in healthy adult hoses was low, consistent with previous reports. Faecal samples were representative for presence of C. difficile in proximal compartments in 5/8 horses (63%) but were not representative for the specific strain.

KW - Faculty of Health and Medical Sciences

KW - Clostridium difficile, Clostridium perfringens, Equine gastrointestinal tract, Equine intestinal microflora, Clostridium difficile ribotyping

KW - Former LIFE faculty

KW - Clostridium difficile

KW - Clostridium perfringens

KW - equine gastrointestinal tract

KW - equine intestinal microflora

KW - Clostridium difficile ribotyping

U2 - 10.1186/1746-6148-8-94

DO - 10.1186/1746-6148-8-94

M3 - Journal article

C2 - 22748233

VL - 8

JO - B M C Veterinary Research

JF - B M C Veterinary Research

SN - 1746-6148

IS - 94

ER -

ID: 40321313