Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS

Research output: Contribution to journalJournal articleResearchpeer-review

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Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS. / Kudirkiene, Egle; Welker, Martin; Knudsen, Nanna Reumert; Bojesen, Anders Miki.

In: Systematic and Applied Microbiology, Vol. 38, No. 5, 23.04.2015, p. 315-322.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kudirkiene, E, Welker, M, Knudsen, NR & Bojesen, AM 2015, 'Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS', Systematic and Applied Microbiology, vol. 38, no. 5, pp. 315-322. https://doi.org/10.1016/j.syapm.2015.02.010

APA

Kudirkiene, E., Welker, M., Knudsen, N. R., & Bojesen, A. M. (2015). Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS. Systematic and Applied Microbiology, 38(5), 315-322. https://doi.org/10.1016/j.syapm.2015.02.010

Vancouver

Kudirkiene E, Welker M, Knudsen NR, Bojesen AM. Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS. Systematic and Applied Microbiology. 2015 Apr 23;38(5):315-322. https://doi.org/10.1016/j.syapm.2015.02.010

Author

Kudirkiene, Egle ; Welker, Martin ; Knudsen, Nanna Reumert ; Bojesen, Anders Miki. / Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS. In: Systematic and Applied Microbiology. 2015 ; Vol. 38, No. 5. pp. 315-322.

Bibtex

@article{db735b4edf9b40838f60b4970a1582f4,
title = "Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS",
abstract = "Streptococcus equi includes very important animal and human pathogens. S. equi subsp. equi (SEE) is a highly pathogenic equine specific subspecies, while S. equi subsp. zooepidemicus (SEZ) and S. equi subsp. ruminatorum are opportunistic pathogens of various animal species and humans. Due to great phenotypic and sequence similarity between three subspecies their discrimination remains difficult. In this study, we aimed to design and validate a novel, Superspectra based, MALDI-TOF MS approach for reliable, rapid and cost-effective identification of SEE and SEZ, the most frequent S. equi subspecies in horses. Superspectra created in this study enabled correct identification of 86 strains belonging to different subspecies of S. equi, isolated from various hosts, infection sites and years. In general, higher average identification accuracy was achieved for SEE (99.0±3.0{\%}) than for SEZ (93.3±7.5{\%}). This result may be attributed to the highly clonal population structure of SEE, as opposed to the diversity of SEZ seen in horses. Importantly strains with atypical colony appearance both within SEE and SEZ did not affect correct identification of the strains by MALDI-TOF MS. Atypical colony variants are often associated with a higher persistence or virulence of S. equi, thus their correct identification using the current method strengthens its potential use in routine clinical diagnostics. In conclusion, reliable identification of S. equi subspecies was achieved by combining a MALDI-TOF MS method with spectra analyses using the SARAMIS database. Additionally, first results on subtyping of SEZ indicated that a more refined discrimination, for example for epidemiological surveys, may be possible",
keywords = "Faculty of Health and Medical Sciences, Streptococcus equi, Horses, Identification, MALDI-TOF MS",
author = "Egle Kudirkiene and Martin Welker and Knudsen, {Nanna Reumert} and Bojesen, {Anders Miki}",
year = "2015",
month = "4",
day = "23",
doi = "10.1016/j.syapm.2015.02.010",
language = "English",
volume = "38",
pages = "315--322",
journal = "Systematic and Applied Microbiology",
issn = "0723-2020",
publisher = "Elsevier GmbH - Urban und Fischer",
number = "5",

}

RIS

TY - JOUR

T1 - Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS

AU - Kudirkiene, Egle

AU - Welker, Martin

AU - Knudsen, Nanna Reumert

AU - Bojesen, Anders Miki

PY - 2015/4/23

Y1 - 2015/4/23

N2 - Streptococcus equi includes very important animal and human pathogens. S. equi subsp. equi (SEE) is a highly pathogenic equine specific subspecies, while S. equi subsp. zooepidemicus (SEZ) and S. equi subsp. ruminatorum are opportunistic pathogens of various animal species and humans. Due to great phenotypic and sequence similarity between three subspecies their discrimination remains difficult. In this study, we aimed to design and validate a novel, Superspectra based, MALDI-TOF MS approach for reliable, rapid and cost-effective identification of SEE and SEZ, the most frequent S. equi subspecies in horses. Superspectra created in this study enabled correct identification of 86 strains belonging to different subspecies of S. equi, isolated from various hosts, infection sites and years. In general, higher average identification accuracy was achieved for SEE (99.0±3.0%) than for SEZ (93.3±7.5%). This result may be attributed to the highly clonal population structure of SEE, as opposed to the diversity of SEZ seen in horses. Importantly strains with atypical colony appearance both within SEE and SEZ did not affect correct identification of the strains by MALDI-TOF MS. Atypical colony variants are often associated with a higher persistence or virulence of S. equi, thus their correct identification using the current method strengthens its potential use in routine clinical diagnostics. In conclusion, reliable identification of S. equi subspecies was achieved by combining a MALDI-TOF MS method with spectra analyses using the SARAMIS database. Additionally, first results on subtyping of SEZ indicated that a more refined discrimination, for example for epidemiological surveys, may be possible

AB - Streptococcus equi includes very important animal and human pathogens. S. equi subsp. equi (SEE) is a highly pathogenic equine specific subspecies, while S. equi subsp. zooepidemicus (SEZ) and S. equi subsp. ruminatorum are opportunistic pathogens of various animal species and humans. Due to great phenotypic and sequence similarity between three subspecies their discrimination remains difficult. In this study, we aimed to design and validate a novel, Superspectra based, MALDI-TOF MS approach for reliable, rapid and cost-effective identification of SEE and SEZ, the most frequent S. equi subspecies in horses. Superspectra created in this study enabled correct identification of 86 strains belonging to different subspecies of S. equi, isolated from various hosts, infection sites and years. In general, higher average identification accuracy was achieved for SEE (99.0±3.0%) than for SEZ (93.3±7.5%). This result may be attributed to the highly clonal population structure of SEE, as opposed to the diversity of SEZ seen in horses. Importantly strains with atypical colony appearance both within SEE and SEZ did not affect correct identification of the strains by MALDI-TOF MS. Atypical colony variants are often associated with a higher persistence or virulence of S. equi, thus their correct identification using the current method strengthens its potential use in routine clinical diagnostics. In conclusion, reliable identification of S. equi subspecies was achieved by combining a MALDI-TOF MS method with spectra analyses using the SARAMIS database. Additionally, first results on subtyping of SEZ indicated that a more refined discrimination, for example for epidemiological surveys, may be possible

KW - Faculty of Health and Medical Sciences

KW - Streptococcus equi

KW - Horses

KW - Identification

KW - MALDI-TOF MS

U2 - 10.1016/j.syapm.2015.02.010

DO - 10.1016/j.syapm.2015.02.010

M3 - Journal article

C2 - 25944783

VL - 38

SP - 315

EP - 322

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

SN - 0723-2020

IS - 5

ER -

ID: 137161943