Reciprocal GLUT-1 and GLUT-4 expression and glucose transport in denervated muscles
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We investigated in 3-day-denervated muscles 1) the expression of GLUT-1 in perineurial sheaths (PNS) and muscle, 2) the muscle fiber-specific changes in GLUT-1 and GLUT-4, and 3) changes in basal and insulin-stimulated 3-O- methylglucose transport. GLUT-1 was increased in both the PNS (P < 0.05) and in the muscle membranes (P < 0.05). GLUT-1 and GLUT-4 concentrations were changed reciprocally, in a fiber-dependent fashion [GLUT-1: red gastrocnemius (RG), + 31%; white gastrocnemius (WG), +10%; GLUT-4: RG, -53%; WG, -16%]. Basal glucose transport was increased (P < 0.05), and this increase was correlated with the oxidative nature of the muscles (r = 0.97). Insulin- stimulated glucose transport was decreased in denervated muscles (P < 0.05). This was also related to the oxidative nature of the muscles (r = -0.88). The increase in basal glucose transport was correlated with the loss of insulin- stimulated transport (r = 0.95). Thus the increase in GLUT-1 compensates for the loss of GLUT-4, resulting in a 56% regain of the reduced insulin- stimulated glucose transport.
Original language | English |
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Journal | American Journal of Physiology - Endocrinology and Metabolism |
Volume | 271 |
Issue number | 1 |
Pages (from-to) | E50-E57 |
Number of pages | 8 |
ISSN | 0193-1849 |
DOIs | |
Publication status | Published - 1996 |
- 3-O-methylglucose transport, hindlimb perfusion, immunofluorescence, insulin, muscle fiber composition, perineurial sheaths, plasma membranes
Research areas
ID: 335353289