Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation. / Basak, Suman; Khare, Harshvardhan Ajay; Roursgaard, Martin; Kempen, Paul J.; Lee, Jong Hyun; Bazban-Shotorbani, Salime; Kraemer, Martin; Chernyy, Sergey; Andresen, Thomas L.; Almdal, Kristoffer; Kamaly, Nazila.

In: Biomacromolecules, Vol. 22, No. 2, 2021, p. 386-398.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Basak, S, Khare, HA, Roursgaard, M, Kempen, PJ, Lee, JH, Bazban-Shotorbani, S, Kraemer, M, Chernyy, S, Andresen, TL, Almdal, K & Kamaly, N 2021, 'Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation', Biomacromolecules, vol. 22, no. 2, pp. 386-398. https://doi.org/10.1021/acs.biomac.0c01238

APA

Basak, S., Khare, H. A., Roursgaard, M., Kempen, P. J., Lee, J. H., Bazban-Shotorbani, S., Kraemer, M., Chernyy, S., Andresen, T. L., Almdal, K., & Kamaly, N. (2021). Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation. Biomacromolecules, 22(2), 386-398. https://doi.org/10.1021/acs.biomac.0c01238

Vancouver

Basak S, Khare HA, Roursgaard M, Kempen PJ, Lee JH, Bazban-Shotorbani S et al. Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation. Biomacromolecules. 2021;22(2):386-398. https://doi.org/10.1021/acs.biomac.0c01238

Author

Basak, Suman ; Khare, Harshvardhan Ajay ; Roursgaard, Martin ; Kempen, Paul J. ; Lee, Jong Hyun ; Bazban-Shotorbani, Salime ; Kraemer, Martin ; Chernyy, Sergey ; Andresen, Thomas L. ; Almdal, Kristoffer ; Kamaly, Nazila. / Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation. In: Biomacromolecules. 2021 ; Vol. 22, No. 2. pp. 386-398.

Bibtex

@article{c2456be2025c43bebf5aebcfc2dede38,
title = "Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation",
abstract = "A key initiating step in atherosclerosis is the accumulation and retention of apolipoprotein B complexing lipoproteins within the artery walls. In this work, we address this exact initiating mechanism of atherosclerosis, which results from the oxidation of low-density lipoproteins (oxLDL) using therapeutic nanogels. We present the development of biocompatible polyethylene glycol (PEG) cross-linked nanogels formed from a single simultaneous cross-linking and co-polymerization step in water without the requirement for an organic solvent, high temperature, or shear stress. The nanogel synthesis also incorporates in situ noncovalent electrostatically driven template polymerization around an innate anti- inflammatory and anti-oxidizing paraoxonase-1 (PON-1) enzyme payload-the release of which is triggered because of matrix metalloproteinase responsive elements instilled in the PEG cross-linker monomer. The results obtained demonstrate the potential of triggered release of the PON-1 enzyme and its efficacy against the production of ox-LDL, and therefore a reduction in macrophage foam cell and reactive oxygen species formation.",
author = "Suman Basak and Khare, {Harshvardhan Ajay} and Martin Roursgaard and Kempen, {Paul J.} and Lee, {Jong Hyun} and Salime Bazban-Shotorbani and Martin Kraemer and Sergey Chernyy and Andresen, {Thomas L.} and Kristoffer Almdal and Nazila Kamaly",
year = "2021",
doi = "10.1021/acs.biomac.0c01238",
language = "English",
volume = "22",
pages = "386--398",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "2",

}

RIS

TY - JOUR

T1 - Simultaneous Cross-Linking and Cross-Polymerization of Enzyme Responsive Polyethylene Glycol Nanogels in Confined Aqueous Droplets for Reduction of Low-Density Lipoprotein Oxidation

AU - Basak, Suman

AU - Khare, Harshvardhan Ajay

AU - Roursgaard, Martin

AU - Kempen, Paul J.

AU - Lee, Jong Hyun

AU - Bazban-Shotorbani, Salime

AU - Kraemer, Martin

AU - Chernyy, Sergey

AU - Andresen, Thomas L.

AU - Almdal, Kristoffer

AU - Kamaly, Nazila

PY - 2021

Y1 - 2021

N2 - A key initiating step in atherosclerosis is the accumulation and retention of apolipoprotein B complexing lipoproteins within the artery walls. In this work, we address this exact initiating mechanism of atherosclerosis, which results from the oxidation of low-density lipoproteins (oxLDL) using therapeutic nanogels. We present the development of biocompatible polyethylene glycol (PEG) cross-linked nanogels formed from a single simultaneous cross-linking and co-polymerization step in water without the requirement for an organic solvent, high temperature, or shear stress. The nanogel synthesis also incorporates in situ noncovalent electrostatically driven template polymerization around an innate anti- inflammatory and anti-oxidizing paraoxonase-1 (PON-1) enzyme payload-the release of which is triggered because of matrix metalloproteinase responsive elements instilled in the PEG cross-linker monomer. The results obtained demonstrate the potential of triggered release of the PON-1 enzyme and its efficacy against the production of ox-LDL, and therefore a reduction in macrophage foam cell and reactive oxygen species formation.

AB - A key initiating step in atherosclerosis is the accumulation and retention of apolipoprotein B complexing lipoproteins within the artery walls. In this work, we address this exact initiating mechanism of atherosclerosis, which results from the oxidation of low-density lipoproteins (oxLDL) using therapeutic nanogels. We present the development of biocompatible polyethylene glycol (PEG) cross-linked nanogels formed from a single simultaneous cross-linking and co-polymerization step in water without the requirement for an organic solvent, high temperature, or shear stress. The nanogel synthesis also incorporates in situ noncovalent electrostatically driven template polymerization around an innate anti- inflammatory and anti-oxidizing paraoxonase-1 (PON-1) enzyme payload-the release of which is triggered because of matrix metalloproteinase responsive elements instilled in the PEG cross-linker monomer. The results obtained demonstrate the potential of triggered release of the PON-1 enzyme and its efficacy against the production of ox-LDL, and therefore a reduction in macrophage foam cell and reactive oxygen species formation.

U2 - 10.1021/acs.biomac.0c01238

DO - 10.1021/acs.biomac.0c01238

M3 - Journal article

C2 - 33125232

VL - 22

SP - 386

EP - 398

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 2

ER -

ID: 258622420