The comprehensive management of ecosystem service benefits and losses provided by landscapes under land-use reorganization is essential for optimizing and regulating landscape patterns and ecological conservation under rapid urbanization. Of the many studies focusing on the quantification and visualization of ecosystem services, few have explored the visualization characteristics of the different scales related to ecosystem services and losses. In this study, we selected one of the most important urban agglomerations in China—the Yangtze River Delta (YRD)—as a case for the analysis of ecosystem service benefits (which include environmental purification, climate and air regulation, and tourism and leisure) and losses (pollen allergies, air pollution, and the heat island effect) and the spatial patterns of both at multiple scales (grid divisions, administrative districts, and functional areas). We found that the value of ecosystem service losses in the YRD, with a net value of approximat