Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects. / Villumsen, Kasper Rømer; Koppang, Erling Olaf; Christensen, Dennis ; Bojesen, Anders Miki.

In: Scientific Reports, Vol. 7, 5930, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Villumsen, KR, Koppang, EO, Christensen, D & Bojesen, AM 2017, 'Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects', Scientific Reports, vol. 7, 5930. https://doi.org/10.1038/s41598-017-06324-7

APA

Villumsen, K. R., Koppang, E. O., Christensen, D., & Bojesen, A. M. (2017). Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects. Scientific Reports, 7, [5930]. https://doi.org/10.1038/s41598-017-06324-7

Vancouver

Villumsen KR, Koppang EO, Christensen D, Bojesen AM. Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects. Scientific Reports. 2017;7. 5930. https://doi.org/10.1038/s41598-017-06324-7

Author

Villumsen, Kasper Rømer ; Koppang, Erling Olaf ; Christensen, Dennis ; Bojesen, Anders Miki. / Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects. In: Scientific Reports. 2017 ; Vol. 7.

Bibtex

@article{bcf2a18a72254fd592c78d9db98118f5,
title = "Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects",
abstract = "In an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. Using rainbow trout (Oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated Aeromonas salmonicida subspecies salmonicida were formulated with CpG oligodeoxynucleotides, the liposomal cationic adjuvant formulation 01 (CAF01) or with Freund’s incomplete adjuvant and administered intraperitoneally. Control groups of unvaccinated, Tris-buffered saline-injected or bacterin-injected individuals were included, and each group included in the study held a total number of 240 individuals. Subsequently, individuals from each group were examined for differences in Fulton’s condition factor, macro- and microscopic pathological changes, as well as protection against experimental infection with A. salmonicida. While adverse effects were not eliminated, reductions in microscopic and macroscopic adverse effects, in particular, were seen for both the nucleotide- and liposome-based vaccine formulations. Furthermore, the induced protection appears similar to that of the benchmark formulation, thus introducing viable, potential alternative types of adjuvants for use in future fish vaccines.",
keywords = "The Faculty of Health and Medical Sciences, akvakultur, forskning, adjuvans, Vaccine, regnbue{\o}rred",
author = "Villumsen, {Kasper R{\o}mer} and Koppang, {Erling Olaf} and Dennis Christensen and Bojesen, {Anders Miki}",
year = "2017",
doi = "10.1038/s41598-017-06324-7",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Alternatives to Mineral Oil Adjuvants in Vaccines Against Aeromonas salmonicida subs. salmonicida in Rainbow Trout Offer Reductions in Adverse Effects

AU - Villumsen, Kasper Rømer

AU - Koppang, Erling Olaf

AU - Christensen, Dennis

AU - Bojesen, Anders Miki

PY - 2017

Y1 - 2017

N2 - In an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. Using rainbow trout (Oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated Aeromonas salmonicida subspecies salmonicida were formulated with CpG oligodeoxynucleotides, the liposomal cationic adjuvant formulation 01 (CAF01) or with Freund’s incomplete adjuvant and administered intraperitoneally. Control groups of unvaccinated, Tris-buffered saline-injected or bacterin-injected individuals were included, and each group included in the study held a total number of 240 individuals. Subsequently, individuals from each group were examined for differences in Fulton’s condition factor, macro- and microscopic pathological changes, as well as protection against experimental infection with A. salmonicida. While adverse effects were not eliminated, reductions in microscopic and macroscopic adverse effects, in particular, were seen for both the nucleotide- and liposome-based vaccine formulations. Furthermore, the induced protection appears similar to that of the benchmark formulation, thus introducing viable, potential alternative types of adjuvants for use in future fish vaccines.

AB - In an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. Using rainbow trout (Oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated Aeromonas salmonicida subspecies salmonicida were formulated with CpG oligodeoxynucleotides, the liposomal cationic adjuvant formulation 01 (CAF01) or with Freund’s incomplete adjuvant and administered intraperitoneally. Control groups of unvaccinated, Tris-buffered saline-injected or bacterin-injected individuals were included, and each group included in the study held a total number of 240 individuals. Subsequently, individuals from each group were examined for differences in Fulton’s condition factor, macro- and microscopic pathological changes, as well as protection against experimental infection with A. salmonicida. While adverse effects were not eliminated, reductions in microscopic and macroscopic adverse effects, in particular, were seen for both the nucleotide- and liposome-based vaccine formulations. Furthermore, the induced protection appears similar to that of the benchmark formulation, thus introducing viable, potential alternative types of adjuvants for use in future fish vaccines.

KW - The Faculty of Health and Medical Sciences

KW - akvakultur, forskning

KW - adjuvans

KW - Vaccine

KW - regnbueørred

U2 - 10.1038/s41598-017-06324-7

DO - 10.1038/s41598-017-06324-7

M3 - Journal article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 5930

ER -

ID: 181354090