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Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing. / Bjorn-Mortensen, K.; Zallet, J.; Lillebaek, T.; Andersen, A. B.; Niemann, S.; Rasmussen, E. M.; Kohl, T. A.
In:
Journal of clinical microbiology, Vol. 53, No. 8, 01.08.2015, p. 2716-2719.
Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
Bjorn-Mortensen, K, Zallet, J
, Lillebaek, T, Andersen, AB, Niemann, S, Rasmussen, EM & Kohl, TA 2015, '
Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing',
Journal of clinical microbiology, vol. 53, no. 8, pp. 2716-2719.
https://doi.org/10.1128/JCM.00662-15APA
Bjorn-Mortensen, K., Zallet, J.
, Lillebaek, T., Andersen, A. B., Niemann, S., Rasmussen, E. M., & Kohl, T. A. (2015).
Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing.
Journal of clinical microbiology,
53(8), 2716-2719.
https://doi.org/10.1128/JCM.00662-15Vancouver
Bjorn-Mortensen K, Zallet J
, Lillebaek T, Andersen AB, Niemann S, Rasmussen EM et al.
Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing.
Journal of clinical microbiology. 2015 Aug 1;53(8):2716-2719.
https://doi.org/10.1128/JCM.00662-15Author
Bjorn-Mortensen, K. ; Zallet, J. ; Lillebaek, T. ; Andersen, A. B. ; Niemann, S. ; Rasmussen, E. M. ; Kohl, T. A. / Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing. In: Journal of clinical microbiology. 2015 ; Vol. 53, No. 8. pp. 2716-2719.
Bibtex
@article{aa25ff01c0e84821bf6d0b34d538a5dc,
title = "Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing",
abstract = "Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.",
author = "K. Bjorn-Mortensen and J. Zallet and T. Lillebaek and Andersen, {A. B.} and S. Niemann and Rasmussen, {E. M.} and Kohl, {T. A.}",
year = "2015",
month = aug,
day = "1",
doi = "10.1128/JCM.00662-15",
language = "English",
volume = "53",
pages = "2716--2719",
journal = "Journal of clinical microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "8",
}
RIS
TY - JOUR
T1 - Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing
AU - Bjorn-Mortensen, K.
AU - Zallet, J.
AU - Lillebaek, T.
AU - Andersen, A. B.
AU - Niemann, S.
AU - Rasmussen, E. M.
AU - Kohl, T. A.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.
AB - Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.
U2 - 10.1128/JCM.00662-15
DO - 10.1128/JCM.00662-15
M3 - Journal article
C2 - 26019203
AN - SCOPUS:84938078200
VL - 53
SP - 2716
EP - 2719
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
SN - 0095-1137
IS - 8
ER -
