Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing
Research output: Contribution to journal › Journal article › Research › peer-review
Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.
Original language | English |
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Journal | Journal of clinical microbiology |
Volume | 53 |
Issue number | 8 |
Pages (from-to) | 2716-2719 |
Number of pages | 4 |
ISSN | 0095-1137 |
DOIs | |
Publication status | Published - 1 Aug 2015 |
ID: 247162981