Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA)

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA). / Bjerrum, L; Glikmann, G; Jensenius, J C; Svehag, S E.

In: Journal of Clinical & Laboratory Immunology, Vol. 10, No. 1, 1983, p. 53-58.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bjerrum, L, Glikmann, G, Jensenius, JC & Svehag, SE 1983, 'Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA)', Journal of Clinical & Laboratory Immunology, vol. 10, no. 1, pp. 53-58.

APA

Bjerrum, L., Glikmann, G., Jensenius, J. C., & Svehag, S. E. (1983). Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA). Journal of Clinical & Laboratory Immunology, 10(1), 53-58.

Vancouver

Bjerrum L, Glikmann G, Jensenius JC, Svehag SE. Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA). Journal of Clinical & Laboratory Immunology. 1983;10(1):53-58.

Author

Bjerrum, L ; Glikmann, G ; Jensenius, J C ; Svehag, S E. / Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA). In: Journal of Clinical & Laboratory Immunology. 1983 ; Vol. 10, No. 1. pp. 53-58.

Bibtex

@article{8028fe2031c611df8ed1000ea68e967b,
title = "Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA)",
abstract = "A microplate-adapted enzyme linked immunosorbent assay (ELISA) for detection of C1q-binding immune complexes (IC) and aggregated IgG (delta IgG) is described. Purified human C1q was adsorbed to the wells of flat-bottomed microtiter plates and EDTA-treated serum samples were subsequently introduced. Bound IC was measured by use of alkaline phosphatase-labelled Protein A followed by the substrate para-nitro-phenyl-phosphate. A dose response was found for both delta IgG and BSA anti-BSA complexes, while variations in the concentration of monomer IgG did not affect the optical density. Elevated levels of IC were found in the majority of sera from patients with rheumatoid arthritis and SLE. The described C1q-PA-ELISA is a simple and inexpensive method for detection of C1q-binding immune complexes. The reproducibility is acceptable and the sensitivity is higher than for most IC-methods based on C1q-binding.",
author = "L Bjerrum and G Glikmann and Jensenius, {J C} and Svehag, {S E}",
year = "1983",
language = "English",
volume = "10",
pages = "53--58",
journal = "Journal of Clinical and Laboratory Immunology",
issn = "0141-2760",
publisher = "Teviot Scientific Publications Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Estimation of immune complexes by a microplate-adapted C1q-Protein A enzyme-linked-immunosorbent-assay (C1q-PA-ELISA)

AU - Bjerrum, L

AU - Glikmann, G

AU - Jensenius, J C

AU - Svehag, S E

PY - 1983

Y1 - 1983

N2 - A microplate-adapted enzyme linked immunosorbent assay (ELISA) for detection of C1q-binding immune complexes (IC) and aggregated IgG (delta IgG) is described. Purified human C1q was adsorbed to the wells of flat-bottomed microtiter plates and EDTA-treated serum samples were subsequently introduced. Bound IC was measured by use of alkaline phosphatase-labelled Protein A followed by the substrate para-nitro-phenyl-phosphate. A dose response was found for both delta IgG and BSA anti-BSA complexes, while variations in the concentration of monomer IgG did not affect the optical density. Elevated levels of IC were found in the majority of sera from patients with rheumatoid arthritis and SLE. The described C1q-PA-ELISA is a simple and inexpensive method for detection of C1q-binding immune complexes. The reproducibility is acceptable and the sensitivity is higher than for most IC-methods based on C1q-binding.

AB - A microplate-adapted enzyme linked immunosorbent assay (ELISA) for detection of C1q-binding immune complexes (IC) and aggregated IgG (delta IgG) is described. Purified human C1q was adsorbed to the wells of flat-bottomed microtiter plates and EDTA-treated serum samples were subsequently introduced. Bound IC was measured by use of alkaline phosphatase-labelled Protein A followed by the substrate para-nitro-phenyl-phosphate. A dose response was found for both delta IgG and BSA anti-BSA complexes, while variations in the concentration of monomer IgG did not affect the optical density. Elevated levels of IC were found in the majority of sera from patients with rheumatoid arthritis and SLE. The described C1q-PA-ELISA is a simple and inexpensive method for detection of C1q-binding immune complexes. The reproducibility is acceptable and the sensitivity is higher than for most IC-methods based on C1q-binding.

M3 - Journal article

C2 - 6338238

VL - 10

SP - 53

EP - 58

JO - Journal of Clinical and Laboratory Immunology

JF - Journal of Clinical and Laboratory Immunology

SN - 0141-2760

IS - 1

ER -

ID: 18686296