Reversibility of exercise-induced translocation of Na+-K+ pump subunits to the plasma membrane in rat skeletal muscle

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C Juel, L Grunnet, M Holse, S Kenworthy, V Sommer, T Wulff

Exercise-induced translocation of Na+-K+ pump subunits to the sarcolemmal membrane was studied using sarcolemmal giant vesicles as a membrane purification procedure. The subunit content was quantified by Western blotting or by ouabain labeling. Low-intensity treadmill running increased (P<0.01) the alpha1, alpha2, beta1, and beta2 subunit contents by 19-32% in membranes from oxidative muscle fibers and the alpha1, alpha2, and beta2 contents increased by 13-25% in membranes from glycolytic muscle fibers. Ouabain labeling of membranes from mixed fibers was increased by 29% after exercise. A similar increase in subunit content could be induced by 5 min of fatiguing, high-intensity electrical stimulation of isolated soleus muscles. An increased subunit content was just detectable in vesicles produced 30 min after exercise, and the content was completely back to control levels 3 h after exercise. It is concluded that both low-intensity long-lasting running and short-lasting high-intensity contractions are able to induce a translocation of pump subunits to the sarcolemmal membrane. The post-exercise disappearance of the extra subunits (half-time approximately 20 min) from the membrane demonstrates the reversible nature of the translocation process.

Original languageEnglish
JournalPflügers Archiv - European Journal of Physiology
Volume443
Issue number2
Pages (from-to)212-7
Number of pages6
ISSN0031-6768
DOIs
Publication statusPublished - Nov 2001

    Research areas

  • Animals, Biological Transport, Cell Membrane/metabolism, Electric Stimulation, Glycolysis, Male, Motor Activity/physiology, Muscle, Skeletal/metabolism, Ouabain/metabolism, Oxidation-Reduction, Rats, Rats, Wistar, Sodium-Potassium-Exchanging ATPase/metabolism, Time Factors

ID: 210980914