Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography. / Møller, Ulla; Keiding, Niels; Larsen, Jørgen Knud.

In: Cell and Tissue Kinetics, Vol. 17, No. 5, 09.1984, p. 497-507.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Møller, U, Keiding, N & Larsen, JK 1984, 'Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography', Cell and Tissue Kinetics, vol. 17, no. 5, pp. 497-507. https://doi.org/10.1111/j.1365-2184.1984.tb00608.x

APA

Møller, U., Keiding, N., & Larsen, J. K. (1984). Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography. Cell and Tissue Kinetics, 17(5), 497-507. https://doi.org/10.1111/j.1365-2184.1984.tb00608.x

Vancouver

Møller U, Keiding N, Larsen JK. Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography. Cell and Tissue Kinetics. 1984 Sep;17(5):497-507. https://doi.org/10.1111/j.1365-2184.1984.tb00608.x

Author

Møller, Ulla ; Keiding, Niels ; Larsen, Jørgen Knud. / Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography. In: Cell and Tissue Kinetics. 1984 ; Vol. 17, No. 5. pp. 497-507.

Bibtex

@article{f99cf868e86f4faa9f37aafb4b684579,
title = "Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography",
abstract = "In the partially synchronized cell system of the hamster cheek pouch epithelium, the inhibitory effect of a bolus injection of methotrexate (Mtx) (2 g/m2, injected at 1200 hr) was analysed by means of both autoradiography and flow cytometry (FCM) in a 21‐hr experiment. For autoradiography [3H]TdR and [3H]UdR were used as tracers for salvage and de nouo pathways of thymidylate (TMP) synthesis, respectively. For FCM no tracers were injected. the autoradiographic studies demonstrated an active TdR salvage pathway for DNA synthesis, not affected by the impaired de novo TMP synthesis. the blocked de novo TMP synthesis was partially released 7 hr after Mtx injection, but it had not totally recovered at the end of the experiment. the decrease in the fraction of S‐phase cells detected about 10 hr after Mtx injection by autoradiographic labelling with [3H]TdR and by FCM was found to be caused by a decrease in the number of cells entering S phase. However, Mtx did not influence the salvage TMP synthesis rate of cells entering S phase.)",
author = "Ulla M{\o}ller and Niels Keiding and Larsen, {J{\o}rgen Knud}",
year = "1984",
month = sep,
doi = "10.1111/j.1365-2184.1984.tb00608.x",
language = "English",
volume = "17",
pages = "497--507",
journal = "Cell and Tissue Kinetics",
issn = "0008-8730",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Effect of Methotrexate On Cells In A Keratinized Epithelium With an Active Thymidine Salvage Pathway Assessed By Autoradiography

AU - Møller, Ulla

AU - Keiding, Niels

AU - Larsen, Jørgen Knud

PY - 1984/9

Y1 - 1984/9

N2 - In the partially synchronized cell system of the hamster cheek pouch epithelium, the inhibitory effect of a bolus injection of methotrexate (Mtx) (2 g/m2, injected at 1200 hr) was analysed by means of both autoradiography and flow cytometry (FCM) in a 21‐hr experiment. For autoradiography [3H]TdR and [3H]UdR were used as tracers for salvage and de nouo pathways of thymidylate (TMP) synthesis, respectively. For FCM no tracers were injected. the autoradiographic studies demonstrated an active TdR salvage pathway for DNA synthesis, not affected by the impaired de novo TMP synthesis. the blocked de novo TMP synthesis was partially released 7 hr after Mtx injection, but it had not totally recovered at the end of the experiment. the decrease in the fraction of S‐phase cells detected about 10 hr after Mtx injection by autoradiographic labelling with [3H]TdR and by FCM was found to be caused by a decrease in the number of cells entering S phase. However, Mtx did not influence the salvage TMP synthesis rate of cells entering S phase.)

AB - In the partially synchronized cell system of the hamster cheek pouch epithelium, the inhibitory effect of a bolus injection of methotrexate (Mtx) (2 g/m2, injected at 1200 hr) was analysed by means of both autoradiography and flow cytometry (FCM) in a 21‐hr experiment. For autoradiography [3H]TdR and [3H]UdR were used as tracers for salvage and de nouo pathways of thymidylate (TMP) synthesis, respectively. For FCM no tracers were injected. the autoradiographic studies demonstrated an active TdR salvage pathway for DNA synthesis, not affected by the impaired de novo TMP synthesis. the blocked de novo TMP synthesis was partially released 7 hr after Mtx injection, but it had not totally recovered at the end of the experiment. the decrease in the fraction of S‐phase cells detected about 10 hr after Mtx injection by autoradiographic labelling with [3H]TdR and by FCM was found to be caused by a decrease in the number of cells entering S phase. However, Mtx did not influence the salvage TMP synthesis rate of cells entering S phase.)

UR - http://www.scopus.com/inward/record.url?scp=84990564060&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2184.1984.tb00608.x

DO - 10.1111/j.1365-2184.1984.tb00608.x

M3 - Journal article

AN - SCOPUS:84990564060

VL - 17

SP - 497

EP - 507

JO - Cell and Tissue Kinetics

JF - Cell and Tissue Kinetics

SN - 0008-8730

IS - 5

ER -

ID: 203013992