Baseline micronuclei frequency in children: estimates from meta- and pooled analyses

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Standard

Baseline micronuclei frequency in children: estimates from meta- and pooled analyses. / Neri, Monica; Ceppi, Marcello; Knudsen, Lisbeth E; Merlo, Domenico Franco; Barale, Roberto; Puntoni, Riccardo; Bonassi, Stefano.

In: Environmental Health Perspectives, Vol. 113, No. 9, 2005, p. 1226-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Neri, M, Ceppi, M, Knudsen, LE, Merlo, DF, Barale, R, Puntoni, R & Bonassi, S 2005, 'Baseline micronuclei frequency in children: estimates from meta- and pooled analyses', Environmental Health Perspectives, vol. 113, no. 9, pp. 1226-9.

APA

Neri, M., Ceppi, M., Knudsen, L. E., Merlo, D. F., Barale, R., Puntoni, R., & Bonassi, S. (2005). Baseline micronuclei frequency in children: estimates from meta- and pooled analyses. Environmental Health Perspectives, 113(9), 1226-9.

Vancouver

Neri M, Ceppi M, Knudsen LE, Merlo DF, Barale R, Puntoni R et al. Baseline micronuclei frequency in children: estimates from meta- and pooled analyses. Environmental Health Perspectives. 2005;113(9):1226-9.

Author

Neri, Monica ; Ceppi, Marcello ; Knudsen, Lisbeth E ; Merlo, Domenico Franco ; Barale, Roberto ; Puntoni, Riccardo ; Bonassi, Stefano. / Baseline micronuclei frequency in children: estimates from meta- and pooled analyses. In: Environmental Health Perspectives. 2005 ; Vol. 113, No. 9. pp. 1226-9.

Bibtex

@article{1a372da0119411df803f000ea68e967b,
title = "Baseline micronuclei frequency in children: estimates from meta- and pooled analyses",
abstract = "The number of studies evaluating the effect of environmental exposure to genotoxic agents in children has rapidly increased in the last few years. The frequency of micronuclei (MN) in peripheral blood lymphocytes determined with the cytokinesis block assay is among the most popular biomarkers used for this purpose, although large inter- and intralaboratory variability of this end point has been observed in population studies. The availability of reference measures is therefore necessary for laboratories to validate protocols and analytical procedures, and for molecular epidemiologists, as well, to estimate the statistical power of studies and to assess the quality of data. In this article, we provide estimates of the baseline frequency of MN in children, conducting a meta-analysis of MN frequency reported by field studies in children and a pooled analysis of individual data [available from published studies and from the Human Micronucleus International Collaborative Study (HUMN) database]. Thirteen articles were selected for meta-analysis, and individual data included in the pooled analysis were retrieved from the databases of 12 laboratories. Overall means of 4.48 [95% confidence interval (CI), 3.35-5.98] and 5.70 (95% CI, 4.29-7.56) MN per 1,000 binucleated cells were estimated by the meta- and pooled analysis, respectively. A clear effect of age was detected, even within the restricted range of pediatric age considered, with significantly lower frequency values in newborns. No influence of sex was found. The study showed the advantage of using data from large collaborative studies and suggested a synergistic use of meta- and pooled analysis.",
author = "Monica Neri and Marcello Ceppi and Knudsen, {Lisbeth E} and Merlo, {Domenico Franco} and Roberto Barale and Riccardo Puntoni and Stefano Bonassi",
note = "Keywords: Adolescent; Child; Child, Preschool; Databases, Factual; Female; Humans; Infant; Infant, Newborn; Lymphocytes; Male; Meta-Analysis as Topic; Micronuclei, Chromosome-Defective; Micronucleus Tests; Reference Values; Reproducibility of Results",
year = "2005",
language = "English",
volume = "113",
pages = "1226--9",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "National Institute of Environmental Health Sciences",
number = "9",

}

RIS

TY - JOUR

T1 - Baseline micronuclei frequency in children: estimates from meta- and pooled analyses

AU - Neri, Monica

AU - Ceppi, Marcello

AU - Knudsen, Lisbeth E

AU - Merlo, Domenico Franco

AU - Barale, Roberto

AU - Puntoni, Riccardo

AU - Bonassi, Stefano

N1 - Keywords: Adolescent; Child; Child, Preschool; Databases, Factual; Female; Humans; Infant; Infant, Newborn; Lymphocytes; Male; Meta-Analysis as Topic; Micronuclei, Chromosome-Defective; Micronucleus Tests; Reference Values; Reproducibility of Results

PY - 2005

Y1 - 2005

N2 - The number of studies evaluating the effect of environmental exposure to genotoxic agents in children has rapidly increased in the last few years. The frequency of micronuclei (MN) in peripheral blood lymphocytes determined with the cytokinesis block assay is among the most popular biomarkers used for this purpose, although large inter- and intralaboratory variability of this end point has been observed in population studies. The availability of reference measures is therefore necessary for laboratories to validate protocols and analytical procedures, and for molecular epidemiologists, as well, to estimate the statistical power of studies and to assess the quality of data. In this article, we provide estimates of the baseline frequency of MN in children, conducting a meta-analysis of MN frequency reported by field studies in children and a pooled analysis of individual data [available from published studies and from the Human Micronucleus International Collaborative Study (HUMN) database]. Thirteen articles were selected for meta-analysis, and individual data included in the pooled analysis were retrieved from the databases of 12 laboratories. Overall means of 4.48 [95% confidence interval (CI), 3.35-5.98] and 5.70 (95% CI, 4.29-7.56) MN per 1,000 binucleated cells were estimated by the meta- and pooled analysis, respectively. A clear effect of age was detected, even within the restricted range of pediatric age considered, with significantly lower frequency values in newborns. No influence of sex was found. The study showed the advantage of using data from large collaborative studies and suggested a synergistic use of meta- and pooled analysis.

AB - The number of studies evaluating the effect of environmental exposure to genotoxic agents in children has rapidly increased in the last few years. The frequency of micronuclei (MN) in peripheral blood lymphocytes determined with the cytokinesis block assay is among the most popular biomarkers used for this purpose, although large inter- and intralaboratory variability of this end point has been observed in population studies. The availability of reference measures is therefore necessary for laboratories to validate protocols and analytical procedures, and for molecular epidemiologists, as well, to estimate the statistical power of studies and to assess the quality of data. In this article, we provide estimates of the baseline frequency of MN in children, conducting a meta-analysis of MN frequency reported by field studies in children and a pooled analysis of individual data [available from published studies and from the Human Micronucleus International Collaborative Study (HUMN) database]. Thirteen articles were selected for meta-analysis, and individual data included in the pooled analysis were retrieved from the databases of 12 laboratories. Overall means of 4.48 [95% confidence interval (CI), 3.35-5.98] and 5.70 (95% CI, 4.29-7.56) MN per 1,000 binucleated cells were estimated by the meta- and pooled analysis, respectively. A clear effect of age was detected, even within the restricted range of pediatric age considered, with significantly lower frequency values in newborns. No influence of sex was found. The study showed the advantage of using data from large collaborative studies and suggested a synergistic use of meta- and pooled analysis.

M3 - Journal article

C2 - 16140632

VL - 113

SP - 1226

EP - 1229

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

IS - 9

ER -

ID: 17398458