DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay. / Møller, P; Wallin, Håkan; Grunnet, N; Risom, L; Knudsen, Lisbeth E.

In: Birth Defects Research, Vol. 18, No. 1, 1998, p. 9-16.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Møller, P, Wallin, H, Grunnet, N, Risom, L & Knudsen, LE 1998, 'DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay', Birth Defects Research, vol. 18, no. 1, pp. 9-16.

APA

Møller, P., Wallin, H., Grunnet, N., Risom, L., & Knudsen, L. E. (1998). DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay. Birth Defects Research, 18(1), 9-16.

Vancouver

Møller P, Wallin H, Grunnet N, Risom L, Knudsen LE. DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay. Birth Defects Research. 1998;18(1):9-16.

Author

Møller, P ; Wallin, Håkan ; Grunnet, N ; Risom, L ; Knudsen, Lisbeth E. / DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay. In: Birth Defects Research. 1998 ; Vol. 18, No. 1. pp. 9-16.

Bibtex

@article{13f3e5f047b811df928f000ea68e967b,
title = "DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay",
abstract = "The induction of DNA damage by commonly used printing ink pigments, C.I. pigment orange 5 (C.I. 12075) and C.I. pigment yellow 12 (C.I. 21090), was investigated in freshly isolated rat hepatocytes with the comet assay. C.I. pigment yellow 12 is a 3,3'-dichlorobenzidine-based diarylide pigment, and C.I. pigment orange 5 is a naphthol-azo pigment. The pigments are virtually insoluble in aqueous solutions, and they have not been tested extensively for toxicological effects. C.I. pigment orange 5 increased the levels of DNA damage at 5 microg/ml (P < 0.02) and C.I. pigment yellow 12 at 20 microg/ml (P < 0.002). The effect of incubation time (20, 40, and 80 min) of the same concentrations of the pigments was tested. The levels of DNA damage were increased up to 80 min. Both pigments produced DNA damage that was in the same range as the food carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. Our data indicate that both C.I. pigment orange 5 and C.I. pigment yellow 12 are genotoxic in hepatocytes with metabolizing capacities. However, further investigation of the metabolism and disposition are required for the evaluation of the safety of these pigments.",
author = "P M{\o}ller and H{\aa}kan Wallin and N Grunnet and L Risom and Knudsen, {Lisbeth E.}",
note = "Keywords: Alkalies; Animals; Azo Compounds; Biotransformation; Cell Separation; Coloring Agents; DNA Damage; Dose-Response Relationship, Drug; Electrophoresis; Female; Liver; Mutagenicity Tests; Quinoxalines; Rats; Rats, Wistar",
year = "1998",
language = "English",
volume = "18",
pages = "9--16",
journal = "Birth Defects Research",
issn = "0270-3211",
publisher = "Wiley",
number = "1",

}

RIS

TY - JOUR

T1 - DNA damage in isolated rat hepatocytes exposed to C.I. pigment orange 5 and C.I. pigment yellow 12 by the alkaline comet assay

AU - Møller, P

AU - Wallin, Håkan

AU - Grunnet, N

AU - Risom, L

AU - Knudsen, Lisbeth E.

N1 - Keywords: Alkalies; Animals; Azo Compounds; Biotransformation; Cell Separation; Coloring Agents; DNA Damage; Dose-Response Relationship, Drug; Electrophoresis; Female; Liver; Mutagenicity Tests; Quinoxalines; Rats; Rats, Wistar

PY - 1998

Y1 - 1998

N2 - The induction of DNA damage by commonly used printing ink pigments, C.I. pigment orange 5 (C.I. 12075) and C.I. pigment yellow 12 (C.I. 21090), was investigated in freshly isolated rat hepatocytes with the comet assay. C.I. pigment yellow 12 is a 3,3'-dichlorobenzidine-based diarylide pigment, and C.I. pigment orange 5 is a naphthol-azo pigment. The pigments are virtually insoluble in aqueous solutions, and they have not been tested extensively for toxicological effects. C.I. pigment orange 5 increased the levels of DNA damage at 5 microg/ml (P < 0.02) and C.I. pigment yellow 12 at 20 microg/ml (P < 0.002). The effect of incubation time (20, 40, and 80 min) of the same concentrations of the pigments was tested. The levels of DNA damage were increased up to 80 min. Both pigments produced DNA damage that was in the same range as the food carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. Our data indicate that both C.I. pigment orange 5 and C.I. pigment yellow 12 are genotoxic in hepatocytes with metabolizing capacities. However, further investigation of the metabolism and disposition are required for the evaluation of the safety of these pigments.

AB - The induction of DNA damage by commonly used printing ink pigments, C.I. pigment orange 5 (C.I. 12075) and C.I. pigment yellow 12 (C.I. 21090), was investigated in freshly isolated rat hepatocytes with the comet assay. C.I. pigment yellow 12 is a 3,3'-dichlorobenzidine-based diarylide pigment, and C.I. pigment orange 5 is a naphthol-azo pigment. The pigments are virtually insoluble in aqueous solutions, and they have not been tested extensively for toxicological effects. C.I. pigment orange 5 increased the levels of DNA damage at 5 microg/ml (P < 0.02) and C.I. pigment yellow 12 at 20 microg/ml (P < 0.002). The effect of incubation time (20, 40, and 80 min) of the same concentrations of the pigments was tested. The levels of DNA damage were increased up to 80 min. Both pigments produced DNA damage that was in the same range as the food carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. Our data indicate that both C.I. pigment orange 5 and C.I. pigment yellow 12 are genotoxic in hepatocytes with metabolizing capacities. However, further investigation of the metabolism and disposition are required for the evaluation of the safety of these pigments.

M3 - Journal article

VL - 18

SP - 9

EP - 16

JO - Birth Defects Research

JF - Birth Defects Research

SN - 0270-3211

IS - 1

ER -

ID: 19231126