Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice

Research output: Contribution to journalJournal articleResearchpeer-review

Astrid Skovmand, Alexander C. Ø. Jensen, Clotilde Maurice, Francesco Marchetti, Anna J. Lauvås, Ismo K. Koponen, Keld A. Jensen, Sandra Goericke-Pesch, Ulla Vogel, Karin S. Hougaard

Background
Previous findings indicate that in utero exposure to nanoparticles may affect the reproductive system in male offspring. Effects such as decreased sperm counts and testicular structural changes in F1 males have been reported following maternal airway exposure to carbon black during gestation. In addition, a previous study in our laboratory suggested that the effects of in utero exposure of nanoparticles may span further than the first generation, as sperm content per gram of testis was significantly lowered in F2 males. In the present study we assessed male fertility parameters following in utero inhalation exposure to carbon black in four generations of mice.

Results
Filter measurements demonstrated that the time-mated females were exposed to a mean total suspended particle mass concentration of 4.79 ± 1.86 or 33.87 ± 14.77 mg/m3 for the low and high exposure, respectively. The control exposure was below the detection limit (LOD 0.08 mg/m3). Exposure did not affect gestation and litter parameters in any generation. No significant changes were observed in body and reproductive organ weights, epididymal sperm parameters, daily sperm production, plasma testosterone or fertility.

Conclusion
In utero exposure to carbon black nanoparticles, at occupationally relevant exposure levels, via maternal whole body inhalation did not affect male-specific reproductive, fertility and litter parameters in four generations of mice.
Original languageEnglish
Article number13
JournalParticle and Fibre Toxicology
Volume16
Number of pages13
ISSN1743-8977
DOIs
Publication statusPublished - 2019

    Research areas

  • Computer assisted sperm analysis, Daily sperm production, In utero, Nanoparticles, Reproductive toxicity, Airway exposure, Sperm quality, Testes

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