Genotoxicity in the absence of inflammation after tungsten inhalation in mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Genotoxicity in the absence of inflammation after tungsten inhalation in mice. / Sørli, Jorid B.; Jensen, Alexander C. Ø.; Mortensen, Alicja; Szarek, Józef; Chatzigianelli, Eleni; Gutierrez, Claudia A.T.; Jacobsen, Nicklas R.; Poulsen, Sarah S.; Hafez, Iosif; Loizides, Charis; Biskos, George; Hougaard, Karin S.; Vogel, Ulla; Hadrup, Niels.

In: Environmental Toxicology and Pharmacology, Vol. 98, 104074, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sørli, JB, Jensen, ACØ, Mortensen, A, Szarek, J, Chatzigianelli, E, Gutierrez, CAT, Jacobsen, NR, Poulsen, SS, Hafez, I, Loizides, C, Biskos, G, Hougaard, KS, Vogel, U & Hadrup, N 2023, 'Genotoxicity in the absence of inflammation after tungsten inhalation in mice', Environmental Toxicology and Pharmacology, vol. 98, 104074. https://doi.org/10.1016/j.etap.2023.104074

APA

Sørli, J. B., Jensen, A. C. Ø., Mortensen, A., Szarek, J., Chatzigianelli, E., Gutierrez, C. A. T., Jacobsen, N. R., Poulsen, S. S., Hafez, I., Loizides, C., Biskos, G., Hougaard, K. S., Vogel, U., & Hadrup, N. (2023). Genotoxicity in the absence of inflammation after tungsten inhalation in mice. Environmental Toxicology and Pharmacology, 98, [104074]. https://doi.org/10.1016/j.etap.2023.104074

Vancouver

Sørli JB, Jensen ACØ, Mortensen A, Szarek J, Chatzigianelli E, Gutierrez CAT et al. Genotoxicity in the absence of inflammation after tungsten inhalation in mice. Environmental Toxicology and Pharmacology. 2023;98. 104074. https://doi.org/10.1016/j.etap.2023.104074

Author

Sørli, Jorid B. ; Jensen, Alexander C. Ø. ; Mortensen, Alicja ; Szarek, Józef ; Chatzigianelli, Eleni ; Gutierrez, Claudia A.T. ; Jacobsen, Nicklas R. ; Poulsen, Sarah S. ; Hafez, Iosif ; Loizides, Charis ; Biskos, George ; Hougaard, Karin S. ; Vogel, Ulla ; Hadrup, Niels. / Genotoxicity in the absence of inflammation after tungsten inhalation in mice. In: Environmental Toxicology and Pharmacology. 2023 ; Vol. 98.

Bibtex

@article{d6c19ba1b12f4d2e9e4eb5172649f1a2,

title = "Genotoxicity in the absence of inflammation after tungsten inhalation in mice",

abstract = "Tungsten is used in several applications and human exposure may occur. To assess its pulmonary toxicity, we exposed male mice to nose-only inhalation of tungsten particles at 9, 23 or 132 mg/m3 (Low, Mid and High exposure) (45 min/day, 5 days/week for 2 weeks). Increased genotoxicity (assessed by comet assay) was seen in bronchoalveolar (BAL) fluid cells at Low and High exposure. We measured acellular ROS production, and cannot exclude that ROS contributed to the observed genotoxicity. We saw no effects on body weight gain, pulmonary inflammation, lactate dehydrogenase or protein in BAL fluid, pathology of liver or kidney, or on sperm counts. In conclusion, tungsten showed non-dose dependent genotoxicity in the absence of inflammation and therefore interpreted to be primary genotoxicity. Based on genotoxicity, a Lowest Observed Adverse Effect Concentration (LOAEC) could be set at 9 mg/m3. It was not possible to establish a No Adverse Effect Concentration (NOAEC).",

keywords = "Comet assay, Pulmonary, Toxicity, Toxicology, Wolfram",

author = "S{\o}rli, {Jorid B.} and Jensen, {Alexander C. {\O}.} and Alicja Mortensen and J{\'o}zef Szarek and Eleni Chatzigianelli and Gutierrez, {Claudia A.T.} and Jacobsen, {Nicklas R.} and Poulsen, {Sarah S.} and Iosif Hafez and Charis Loizides and George Biskos and Hougaard, {Karin S.} and Ulla Vogel and Niels Hadrup",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

doi = "10.1016/j.etap.2023.104074",

language = "English",

volume = "98",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Genotoxicity in the absence of inflammation after tungsten inhalation in mice

AU - Sørli, Jorid B.

AU - Jensen, Alexander C. Ø.

AU - Mortensen, Alicja

AU - Szarek, Józef

AU - Chatzigianelli, Eleni

AU - Gutierrez, Claudia A.T.

AU - Jacobsen, Nicklas R.

AU - Poulsen, Sarah S.

AU - Hafez, Iosif

AU - Loizides, Charis

AU - Biskos, George

AU - Hougaard, Karin S.

AU - Vogel, Ulla

AU - Hadrup, Niels

PY - 2023

Y1 - 2023

N2 - Tungsten is used in several applications and human exposure may occur. To assess its pulmonary toxicity, we exposed male mice to nose-only inhalation of tungsten particles at 9, 23 or 132 mg/m3 (Low, Mid and High exposure) (45 min/day, 5 days/week for 2 weeks). Increased genotoxicity (assessed by comet assay) was seen in bronchoalveolar (BAL) fluid cells at Low and High exposure. We measured acellular ROS production, and cannot exclude that ROS contributed to the observed genotoxicity. We saw no effects on body weight gain, pulmonary inflammation, lactate dehydrogenase or protein in BAL fluid, pathology of liver or kidney, or on sperm counts. In conclusion, tungsten showed non-dose dependent genotoxicity in the absence of inflammation and therefore interpreted to be primary genotoxicity. Based on genotoxicity, a Lowest Observed Adverse Effect Concentration (LOAEC) could be set at 9 mg/m3. It was not possible to establish a No Adverse Effect Concentration (NOAEC).

AB - Tungsten is used in several applications and human exposure may occur. To assess its pulmonary toxicity, we exposed male mice to nose-only inhalation of tungsten particles at 9, 23 or 132 mg/m3 (Low, Mid and High exposure) (45 min/day, 5 days/week for 2 weeks). Increased genotoxicity (assessed by comet assay) was seen in bronchoalveolar (BAL) fluid cells at Low and High exposure. We measured acellular ROS production, and cannot exclude that ROS contributed to the observed genotoxicity. We saw no effects on body weight gain, pulmonary inflammation, lactate dehydrogenase or protein in BAL fluid, pathology of liver or kidney, or on sperm counts. In conclusion, tungsten showed non-dose dependent genotoxicity in the absence of inflammation and therefore interpreted to be primary genotoxicity. Based on genotoxicity, a Lowest Observed Adverse Effect Concentration (LOAEC) could be set at 9 mg/m3. It was not possible to establish a No Adverse Effect Concentration (NOAEC).

KW - Comet assay

KW - Pulmonary

KW - Toxicity

KW - Toxicology

KW - Wolfram

U2 - 10.1016/j.etap.2023.104074

DO - 10.1016/j.etap.2023.104074

M3 - Journal article

C2 - 36724834

AN - SCOPUS:85147277179

VL - 98

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

SN - 1382-6689

M1 - 104074

ER -

ID: 338050073

Department of Public Health