Inhalation of hydrogenated vegetable oil combustion exhaust and genotoxicity responses in humans
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Inhalation of hydrogenated vegetable oil combustion exhaust and genotoxicity responses in humans. / Scholten, Rebecca Harnung; Essig, Yona J.; Roursgaard, Martin; Jensen, Annie; Krais, Annette M.; Gren, Louise; Dierschke, Katrin; Gudmundsson, Anders; Wierzbicka, Aneta; Moller, Peter.
In: Archives of Toxicology, Vol. 95, 2021, p. 3407–3416.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Inhalation of hydrogenated vegetable oil combustion exhaust and genotoxicity responses in humans
AU - Scholten, Rebecca Harnung
AU - Essig, Yona J.
AU - Roursgaard, Martin
AU - Jensen, Annie
AU - Krais, Annette M.
AU - Gren, Louise
AU - Dierschke, Katrin
AU - Gudmundsson, Anders
AU - Wierzbicka, Aneta
AU - Moller, Peter
PY - 2021
Y1 - 2021
N2 - Biofuels from vegetable oils or animal fats are considered to be more sustainable than petroleum-derived diesel fuel. In this study, we have assessed the effect of hydrogenated vegetable oil (HVO) exhaust on levels of DNA damage in peripheral blood mononuclear cells (PBMCs) as primary outcome, and oxidative stress and inflammation as mediators of genotoxicity. In a randomized cross-over study, healthy humans were exposed to filtered air, inorganic salt particles, exhausts from combustion of HVO in engines with aftertreatment [i.e. emission with nitrogen oxides and low amounts of particulate matter less than 2.5 mu m (approximately 1 mu g/m(3))], or without aftertreatment (i.e. emission with nitrogen oxides and 93 +/- 13 mu g/m(3) of PM2.5). The subjects were exposed for 3 h and blood samples were collected before, within 1 h after the exposure and 24 h after. None of the exposures caused generation of DNA strand breaks and oxidatively damaged DNA, or affected gene expression of factors related to DNA repair (Ogg1), antioxidant defense (Hmox1) or pro-inflammatory cytokines (Ccl2, Il8 and Tnfa) in PBMCs. The results from this study indicate that short-term HVO exhaust exposure is not associated with genotoxic hazard in humans.
AB - Biofuels from vegetable oils or animal fats are considered to be more sustainable than petroleum-derived diesel fuel. In this study, we have assessed the effect of hydrogenated vegetable oil (HVO) exhaust on levels of DNA damage in peripheral blood mononuclear cells (PBMCs) as primary outcome, and oxidative stress and inflammation as mediators of genotoxicity. In a randomized cross-over study, healthy humans were exposed to filtered air, inorganic salt particles, exhausts from combustion of HVO in engines with aftertreatment [i.e. emission with nitrogen oxides and low amounts of particulate matter less than 2.5 mu m (approximately 1 mu g/m(3))], or without aftertreatment (i.e. emission with nitrogen oxides and 93 +/- 13 mu g/m(3) of PM2.5). The subjects were exposed for 3 h and blood samples were collected before, within 1 h after the exposure and 24 h after. None of the exposures caused generation of DNA strand breaks and oxidatively damaged DNA, or affected gene expression of factors related to DNA repair (Ogg1), antioxidant defense (Hmox1) or pro-inflammatory cytokines (Ccl2, Il8 and Tnfa) in PBMCs. The results from this study indicate that short-term HVO exhaust exposure is not associated with genotoxic hazard in humans.
KW - Biofuel
KW - DNA damage
KW - Oxidative stress
KW - Inflammation
KW - Genotoxicity
KW - Aerosol
KW - Controlled human exposure
KW - OXIDATIVELY DAMAGED DNA
KW - 2ND-GENERATION BIODIESEL FUELS
KW - LUNG LAVAGE FLUIDS
KW - DIESEL EXHAUST
KW - AIR-POLLUTION
KW - ULTRAFINE PARTICLES
KW - PARTICULATE MATTER
KW - EMISSION PARTICLES
KW - EPITHELIAL-CELLS
KW - METHYL-ESTER
U2 - 10.1007/s00204-021-03143-8
DO - 10.1007/s00204-021-03143-8
M3 - Journal article
C2 - 34468814
VL - 95
SP - 3407
EP - 3416
JO - Archives of Toxicology
JF - Archives of Toxicology
SN - 0340-5761
ER -
ID: 279381314