Oxidative stress, inflammation, and DNA damage in rats after intratracheal instillation or oral exposure to ambient air and wood smoke particulate matter
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Oxidative stress, inflammation, and DNA damage in rats after intratracheal instillation or oral exposure to ambient air and wood smoke particulate matter. / Danielsen, Pernille Høgh; Loft, Steffen; Jacobsen, Nicklas Raun; Jensen, Keld Alstrup; Autrup, Herman; Ravanat, Jean-Luc; Wallin, Håkan; Møller, Peter.
In: Toxicological Sciences, Vol. 118, No. 2, 01.12.2010, p. 574-85.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Oxidative stress, inflammation, and DNA damage in rats after intratracheal instillation or oral exposure to ambient air and wood smoke particulate matter
AU - Danielsen, Pernille Høgh
AU - Loft, Steffen
AU - Jacobsen, Nicklas Raun
AU - Jensen, Keld Alstrup
AU - Autrup, Herman
AU - Ravanat, Jean-Luc
AU - Wallin, Håkan
AU - Møller, Peter
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Wood combustion is a significant source of ambient particulate matter (PM) in many regions of the world. Exposure occurs through inhalation or ingestion after deposition of wood smoke particulate matter (WSPM) on crops and food. We investigated effects of ambient PM and WSPM by intragastric or intratracheal exposure in terms of oxidative stress, inflammation, genotoxicity, and DNA repair after 24 h in liver and lung tissue of rats. Rats were exposed to WSPM from high or low oxygen combustion and ambient PM collected in areas with and without many operating wood stoves or carbon black (CB) at the dose of 0.64 mg/kg body weight. The levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine, 1,N(6)-etheno-2'-deoxyadenosine, and 1-N(2)-etheno-2'-deoxyguanosine (edG) were significantly increased with 23% (95% confidence interval [CI]: 0.1-45%), 54% (95% CI:18-90%), and 73% (95% CI: 31-134%) in the liver of rats exposed orally to CB, respectively. Rats orally exposed to PM from the wood stove area and low oxygen combustion WSPM (LOWS) had 35% (95% CI: 0.1-71%) and 45% (95% CI: 10-82%) increased levels of edG in the liver, respectively. No significant differences were observed for bulky DNA adducts. Increased gene expression of proinflammatory cytokines, heme oxygenase-1, and oxoguanine DNA glycosylase 1 was observed in the liver following intragastric exposure and in the lung following instillation in particular of LOWS. Exposure to LOWS also increased the proportion of neutrophils in BAL fluid. These results indicate that WSPM and CB exert the strongest effect in terms of oxidative stress-induced response, inflammation, and genotoxicity in the organ closest to the port of entry.
AB - Wood combustion is a significant source of ambient particulate matter (PM) in many regions of the world. Exposure occurs through inhalation or ingestion after deposition of wood smoke particulate matter (WSPM) on crops and food. We investigated effects of ambient PM and WSPM by intragastric or intratracheal exposure in terms of oxidative stress, inflammation, genotoxicity, and DNA repair after 24 h in liver and lung tissue of rats. Rats were exposed to WSPM from high or low oxygen combustion and ambient PM collected in areas with and without many operating wood stoves or carbon black (CB) at the dose of 0.64 mg/kg body weight. The levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine, 1,N(6)-etheno-2'-deoxyadenosine, and 1-N(2)-etheno-2'-deoxyguanosine (edG) were significantly increased with 23% (95% confidence interval [CI]: 0.1-45%), 54% (95% CI:18-90%), and 73% (95% CI: 31-134%) in the liver of rats exposed orally to CB, respectively. Rats orally exposed to PM from the wood stove area and low oxygen combustion WSPM (LOWS) had 35% (95% CI: 0.1-71%) and 45% (95% CI: 10-82%) increased levels of edG in the liver, respectively. No significant differences were observed for bulky DNA adducts. Increased gene expression of proinflammatory cytokines, heme oxygenase-1, and oxoguanine DNA glycosylase 1 was observed in the liver following intragastric exposure and in the lung following instillation in particular of LOWS. Exposure to LOWS also increased the proportion of neutrophils in BAL fluid. These results indicate that WSPM and CB exert the strongest effect in terms of oxidative stress-induced response, inflammation, and genotoxicity in the organ closest to the port of entry.
KW - Administration, Oral
KW - Animals
KW - Biological Markers
KW - Bronchoalveolar Lavage Fluid
KW - Cell Count
KW - Chemokine CCL2
KW - Chemokine CXCL2
KW - Cytokines
KW - DNA Adducts
KW - DNA Damage
KW - DNA Glycosylases
KW - Gene Expression
KW - Heme Oxygenase-1
KW - Intubation, Intratracheal
KW - Male
KW - Membrane Proteins
KW - Oxidative Stress
KW - Particulate Matter
KW - Pneumonia
KW - Rats
KW - Rats, Inbred F344
KW - Smoke
KW - Wood
U2 - 10.1093/toxsci/kfq290
DO - 10.1093/toxsci/kfq290
M3 - Journal article
C2 - 20864625
VL - 118
SP - 574
EP - 585
JO - Toxicological Sciences
JF - Toxicological Sciences
SN - 1096-6080
IS - 2
ER -
ID: 33595655