Regional blood flow during exercise in humans measured by near-infrared spectroscopy and indocyanine green
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Regional blood flow during exercise in humans measured by near-infrared spectroscopy and indocyanine green. / Boushel, R; Langberg, Henning; Olesen, J; Nowak, M; Simonsen, L; Bülow, J; Kjaer, M.
In: Journal of Applied Physiology, Vol. 89, No. 5, 11.2000, p. 1868-78.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Regional blood flow during exercise in humans measured by near-infrared spectroscopy and indocyanine green
AU - Boushel, R
AU - Langberg, Henning
AU - Olesen, J
AU - Nowak, M
AU - Simonsen, L
AU - Bülow, J
AU - Kjaer, M
PY - 2000/11
Y1 - 2000/11
N2 - Using near-infrared spectroscopy (NIRS) and the tracer indocyanine green (ICG), we quantified blood flow in calf muscle and around the Achilles tendon during plantar flexion (1-9 W). For comparison, blood flow in calf muscle was determined by dye dilution in combination with magnetic resonance imaging measures of muscle volume, and, for the peritendon region, blood flow was measured by (133)Xe washout. From rest to a peak load of 9 W, NIRS-ICG blood flow in calf muscle increased from 2.4+/-0.2 to 74+/-5 ml x 100 ml tissue(-1) x min(-1), similar to that measured by reverse dye (77+/-6 ml x 100 ml tissue(-1) x min(-1)). Achilles peritendon blood flow measured by NIRS-ICG rose with exercise from 2.2+/-0.5 to 15.1+/-0.2 ml x 100 ml(-1) x min(-1), which was similar to that determined by (133)Xe washout (2.0+/-0.6 to 14.6+/-0.3 ml x 100 ml tissue(-1) x min(-1)). This is the first study using NIRS and ICG to quantify regional tissue blood flow during exercise in humans. Due to its high spatial and temporal resolution, the technique may be useful for determining regional blood flow distribution and regulation during exercise in humans.
AB - Using near-infrared spectroscopy (NIRS) and the tracer indocyanine green (ICG), we quantified blood flow in calf muscle and around the Achilles tendon during plantar flexion (1-9 W). For comparison, blood flow in calf muscle was determined by dye dilution in combination with magnetic resonance imaging measures of muscle volume, and, for the peritendon region, blood flow was measured by (133)Xe washout. From rest to a peak load of 9 W, NIRS-ICG blood flow in calf muscle increased from 2.4+/-0.2 to 74+/-5 ml x 100 ml tissue(-1) x min(-1), similar to that measured by reverse dye (77+/-6 ml x 100 ml tissue(-1) x min(-1)). Achilles peritendon blood flow measured by NIRS-ICG rose with exercise from 2.2+/-0.5 to 15.1+/-0.2 ml x 100 ml(-1) x min(-1), which was similar to that determined by (133)Xe washout (2.0+/-0.6 to 14.6+/-0.3 ml x 100 ml tissue(-1) x min(-1)). This is the first study using NIRS and ICG to quantify regional tissue blood flow during exercise in humans. Due to its high spatial and temporal resolution, the technique may be useful for determining regional blood flow distribution and regulation during exercise in humans.
KW - Coloring Agents
KW - Exercise
KW - Humans
KW - Indocyanine Green
KW - Leg
KW - Muscle, Skeletal
KW - Regional Blood Flow
KW - Sensitivity and Specificity
KW - Spectroscopy, Near-Infrared
KW - Xenon Radioisotopes
M3 - Journal article
C2 - 11053338
VL - 89
SP - 1868
EP - 1878
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 5
ER -
ID: 98570611