Substantial elevation of interleukin-6 concentration in peritendinous tissue, in contrast to muscle, following prolonged exercise in humans
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Substantial elevation of interleukin-6 concentration in peritendinous tissue, in contrast to muscle, following prolonged exercise in humans. / Langberg, Henning; Olesen, Jens; Gemmer, Carsten; Kjaer, Michael.
In: Journal of Physiology, Vol. 542, No. Pt 3, 2002, p. 985-90.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Substantial elevation of interleukin-6 concentration in peritendinous tissue, in contrast to muscle, following prolonged exercise in humans
AU - Langberg, Henning
AU - Olesen, Jens
AU - Gemmer, Carsten
AU - Kjaer, Michael
PY - 2002
Y1 - 2002
N2 - Plasma interleukin-6 (IL-6) concentration has been shown to increase with exercise and various cell types and tissues have been suggested to be responsible for this increase. At present no studies have measured the interstitial concentration of IL-6 in skeletal muscle and connective tissue. The present study represents the first attempt to simultaneously measure IL-6 in plasma, skeletal muscle and peritendinous connective tissue in response to prolonged exercise. Six healthy well-trained volunteers completed a 36 km run (flat, 12 km h(-1)). IL-6 was measured before, 2 h post-exercise and 24 h, 48 h, 72 h and 96 h post-exercise in both the medial gastrocnemius muscle (not measured at rest due to risk of disabling the subsequent exercise, and 24 h and 72 h post-exercise) and the peritendinous tissue around the Achilles tendon using microdialysis catheters with a high molecular mass cut-off value (3000 kDa). The plasma concentration of IL-6 was measured simultaneously, and in addition every hour during the exercise, by enzyme-linked immunosorbent assay (ELISA). The plasma concentration of IL-6 was found to increase throughout the exercise, reaching peak values immediately after completion of the run (50-fold increase). Using the microdialysis technique, the interstitial concentration of IL-6 was found to increase dramatically from 0 +/- 0 pg ml(-1) to 3618 +/- 1239 pg ml(-1) in the peritendinous tissue in the hours following the exercise. The pattern of changes was similar in plasma and peritendinous tissue, although approximately 100-fold higher in the latter. For comparison the interstitial muscle concentration was found to be 465 +/- 176 pg ml(-1) when measured 2 h post-exercise and 223 +/- 113 pg ml(-1) and 198 +/- 96 pg ml(-1) 48 h and 96 h post-exercise, respectively. The present study demonstrates that the connective tissue around the human Achilles tendon produces significant amounts of IL-6 in response to prolonged physical activity, which might contribute to the exercise-induced increase in IL-6 found in plasma.
AB - Plasma interleukin-6 (IL-6) concentration has been shown to increase with exercise and various cell types and tissues have been suggested to be responsible for this increase. At present no studies have measured the interstitial concentration of IL-6 in skeletal muscle and connective tissue. The present study represents the first attempt to simultaneously measure IL-6 in plasma, skeletal muscle and peritendinous connective tissue in response to prolonged exercise. Six healthy well-trained volunteers completed a 36 km run (flat, 12 km h(-1)). IL-6 was measured before, 2 h post-exercise and 24 h, 48 h, 72 h and 96 h post-exercise in both the medial gastrocnemius muscle (not measured at rest due to risk of disabling the subsequent exercise, and 24 h and 72 h post-exercise) and the peritendinous tissue around the Achilles tendon using microdialysis catheters with a high molecular mass cut-off value (3000 kDa). The plasma concentration of IL-6 was measured simultaneously, and in addition every hour during the exercise, by enzyme-linked immunosorbent assay (ELISA). The plasma concentration of IL-6 was found to increase throughout the exercise, reaching peak values immediately after completion of the run (50-fold increase). Using the microdialysis technique, the interstitial concentration of IL-6 was found to increase dramatically from 0 +/- 0 pg ml(-1) to 3618 +/- 1239 pg ml(-1) in the peritendinous tissue in the hours following the exercise. The pattern of changes was similar in plasma and peritendinous tissue, although approximately 100-fold higher in the latter. For comparison the interstitial muscle concentration was found to be 465 +/- 176 pg ml(-1) when measured 2 h post-exercise and 223 +/- 113 pg ml(-1) and 198 +/- 96 pg ml(-1) 48 h and 96 h post-exercise, respectively. The present study demonstrates that the connective tissue around the human Achilles tendon produces significant amounts of IL-6 in response to prolonged physical activity, which might contribute to the exercise-induced increase in IL-6 found in plasma.
KW - Achilles Tendon
KW - Adult
KW - Connective Tissue
KW - Exercise
KW - Humans
KW - Interleukin-6
KW - Male
KW - Muscle, Skeletal
KW - Osmolar Concentration
KW - Running
KW - Time Factors
M3 - Journal article
C2 - 12154195
VL - 542
SP - 985
EP - 990
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - Pt 3
ER -
ID: 38366886