The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio

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The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio. / Holm, Anja; Possovre, Marie Laure; Bandarabadi, Mojtaba; Moseholm, Kristine F.; Justinussen, Jessica L.; Bozic, Ivan; Lemcke, René; Arribat, Yoan; Amati, Francesca; Silahtaroglu, Asli; Juventin, Maxime; Adamantidis, Antoine; Tafti, Mehdi; Kornum, Birgitte R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 119, No. 17, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Holm, A, Possovre, ML, Bandarabadi, M, Moseholm, KF, Justinussen, JL, Bozic, I, Lemcke, R, Arribat, Y, Amati, F, Silahtaroglu, A, Juventin, M, Adamantidis, A, Tafti, M & Kornum, BR 2022, 'The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 17. https://doi.org/10.1073/pnas.2112225119

APA

Holm, A., Possovre, M. L., Bandarabadi, M., Moseholm, K. F., Justinussen, J. L., Bozic, I., Lemcke, R., Arribat, Y., Amati, F., Silahtaroglu, A., Juventin, M., Adamantidis, A., Tafti, M., & Kornum, B. R. (2022). The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio. Proceedings of the National Academy of Sciences of the United States of America, 119(17). https://doi.org/10.1073/pnas.2112225119

Vancouver

Holm A, Possovre ML, Bandarabadi M, Moseholm KF, Justinussen JL, Bozic I et al. The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio. Proceedings of the National Academy of Sciences of the United States of America. 2022;119(17). https://doi.org/10.1073/pnas.2112225119

Author

Holm, Anja ; Possovre, Marie Laure ; Bandarabadi, Mojtaba ; Moseholm, Kristine F. ; Justinussen, Jessica L. ; Bozic, Ivan ; Lemcke, René ; Arribat, Yoan ; Amati, Francesca ; Silahtaroglu, Asli ; Juventin, Maxime ; Adamantidis, Antoine ; Tafti, Mehdi ; Kornum, Birgitte R. / The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio. In: Proceedings of the National Academy of Sciences of the United States of America. 2022 ; Vol. 119, No. 17.

Bibtex

@article{dfa490f223ce4c839cdfba11aad05583,
title = "The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio",
abstract = "SignificanceThe hypocretin (Hcrt, also known as orexin) neuropeptides regulate sleep and wake stability, and disturbances of Hcrt can lead to sleep disorders. MicroRNAs (miRNAs) are short noncoding RNAs that fine-tune protein expression levels, and miRNA-based therapeutics are emerging. We report a functional interaction between miRNA (miR-137) and Hcrt. We demonstrate that intracellular miR-137 levels in Hcrt neurons regulate Hcrt expression with downstream effects on wakefulness. Specifically, lowering of miR-137 levels increased wakefulness in mice. We further show that the miR-137:Hcrt interaction is conserved across mice and humans, that miR-137 also regulates sleep-wake balance in zebrafish, and that the MIR137 locus is genetically associated with sleep duration in humans. Together, our findings reveal an evolutionarily conserved sleep-wake regulatory role of miR-137.",
keywords = "hypocretin, miR-137, orexin, sleep, wake",
author = "Anja Holm and Possovre, {Marie Laure} and Mojtaba Bandarabadi and Moseholm, {Kristine F.} and Justinussen, {Jessica L.} and Ivan Bozic and Ren{\'e} Lemcke and Yoan Arribat and Francesca Amati and Asli Silahtaroglu and Maxime Juventin and Antoine Adamantidis and Mehdi Tafti and Kornum, {Birgitte R.}",
year = "2022",
doi = "10.1073/pnas.2112225119",
language = "English",
volume = "119",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "17",

}

RIS

TY - JOUR

T1 - The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio

AU - Holm, Anja

AU - Possovre, Marie Laure

AU - Bandarabadi, Mojtaba

AU - Moseholm, Kristine F.

AU - Justinussen, Jessica L.

AU - Bozic, Ivan

AU - Lemcke, René

AU - Arribat, Yoan

AU - Amati, Francesca

AU - Silahtaroglu, Asli

AU - Juventin, Maxime

AU - Adamantidis, Antoine

AU - Tafti, Mehdi

AU - Kornum, Birgitte R.

PY - 2022

Y1 - 2022

N2 - SignificanceThe hypocretin (Hcrt, also known as orexin) neuropeptides regulate sleep and wake stability, and disturbances of Hcrt can lead to sleep disorders. MicroRNAs (miRNAs) are short noncoding RNAs that fine-tune protein expression levels, and miRNA-based therapeutics are emerging. We report a functional interaction between miRNA (miR-137) and Hcrt. We demonstrate that intracellular miR-137 levels in Hcrt neurons regulate Hcrt expression with downstream effects on wakefulness. Specifically, lowering of miR-137 levels increased wakefulness in mice. We further show that the miR-137:Hcrt interaction is conserved across mice and humans, that miR-137 also regulates sleep-wake balance in zebrafish, and that the MIR137 locus is genetically associated with sleep duration in humans. Together, our findings reveal an evolutionarily conserved sleep-wake regulatory role of miR-137.

AB - SignificanceThe hypocretin (Hcrt, also known as orexin) neuropeptides regulate sleep and wake stability, and disturbances of Hcrt can lead to sleep disorders. MicroRNAs (miRNAs) are short noncoding RNAs that fine-tune protein expression levels, and miRNA-based therapeutics are emerging. We report a functional interaction between miRNA (miR-137) and Hcrt. We demonstrate that intracellular miR-137 levels in Hcrt neurons regulate Hcrt expression with downstream effects on wakefulness. Specifically, lowering of miR-137 levels increased wakefulness in mice. We further show that the miR-137:Hcrt interaction is conserved across mice and humans, that miR-137 also regulates sleep-wake balance in zebrafish, and that the MIR137 locus is genetically associated with sleep duration in humans. Together, our findings reveal an evolutionarily conserved sleep-wake regulatory role of miR-137.

KW - hypocretin

KW - miR-137

KW - orexin

KW - sleep

KW - wake

U2 - 10.1073/pnas.2112225119

DO - 10.1073/pnas.2112225119

M3 - Journal article

C2 - 35452310

AN - SCOPUS:85129778441

VL - 119

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 17

ER -

ID: 306698128