Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

Research output: Contribution to journalJournal articleResearchpeer-review

200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.
Original languageEnglish
JournalScandinavian Journal of Infectious Diseases
Issue number3
Pages (from-to)283-5
Number of pages3
Publication statusPublished - 1 Jan 1990

    Research areas

  • Blotting, Western, Enzyme-Linked Immunosorbent Assay, False Negative Reactions, False Positive Reactions, HIV Antibodies, HIV Infections, Humans, Observer Variation, Predictive Value of Tests, Tanzania

ID: 33890974