In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines
Research output: Contribution to journal › Journal article › peer-review
Standard
In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines. / Roursgaard, Martin; Knudsen, Kristina Bram; Northeved, Helle; Persson, Mikael; Christensen, Tina; Kumar, Pramod E. K.; Permin, Anders; Andresen, Thomas L.; Gjetting, Torben; Lykkesfeldt, Jens; Vesterdal, Lise K.; Loft, Steffen; Møller, Peter.
In: Toxicology in Vitro, Vol. 36, 10.2016, p. 164-171.Research output: Contribution to journal › Journal article › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines
AU - Roursgaard, Martin
AU - Knudsen, Kristina Bram
AU - Northeved, Helle
AU - Persson, Mikael
AU - Christensen, Tina
AU - Kumar, Pramod E. K.
AU - Permin, Anders
AU - Andresen, Thomas L.
AU - Gjetting, Torben
AU - Lykkesfeldt, Jens
AU - Vesterdal, Lise K.
AU - Loft, Steffen
AU - Møller, Peter
N1 - Copyright © 2016 Elsevier Ltd. All rights reserved.
PY - 2016/10
Y1 - 2016/10
N2 - The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.
AB - The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.
KW - Journal Article
U2 - 10.1016/j.tiv.2016.08.002
DO - 10.1016/j.tiv.2016.08.002
M3 - Journal article
C2 - 27497994
VL - 36
SP - 164
EP - 171
JO - Toxicology in Vitro
JF - Toxicology in Vitro
SN - 0887-2333
ER -
ID: 165568160