In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines

Research output: Contribution to journalJournal articleResearchpeer-review

Martin Roursgaard, Kristina Bram Knudsen, Helle Northeved, Mikael Persson, Tina Christensen, Pramod E. K. Kumar, Anders Permin, Thomas L. Andresen, Torben Gjetting, Jens Lykkesfeldt, Lise K. Vesterdal, Steffen Loft, Peter Møller

The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.

Original languageEnglish
JournalToxicology in Vitro
Volume36
Pages (from-to)164-171
Number of pages8
ISSN0887-2333
DOIs
Publication statusPublished - Oct 2016

    Research areas

  • Journal Article

ID: 165568160