Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine

Research output: Contribution to journalJournal articleResearchpeer-review

Lars Barregard, Peter Møller, Trine Henriksen, Vilas Mistry, Gudrun Koppen, Pavel Rossner, Radim J Sram, Allan Weimann, Henrik E Poulsen, Robert Nataf, Roberta Andreoli, Paola Manini, Tim Marczylo, Patricia Lam, Mark D Evans, Hiroshi Kasai, Kazuaki Kawai, Yun-Shan Li, Kazuo Sakai, Rajinder Singh & 24 others Friederike Teichert, Peter B Farmer, Rafal Rozalski, Daniel Gackowski, Agnieszka Siomek, Guillermo T Saez, Concha Cerda, Karin Broberg, Christian Lindh, Mohammad Bakhtiar Hossain, Siamak Haghdoost, Chiung-Wen Hu, Mu-Rong Chao, Kuen-Yuh Wu, Hilmi Orhan, Nilufer Senduran, Raymond J Smith, Regina M Santella, Yali Su, Czarina Cortez, Susan Yeh, Ryszard Olinski, Steffen Loft, Marcus S Cooke

Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.
Original languageEnglish
JournalAntioxidants & Redox Signaling
Volume18
Issue number18
Pages (from-to)2377-91
Number of pages15
ISSN1523-0864
DOIs
Publication statusPublished - 20 Jun 2013

    Research areas

  • Adult, Artifacts, Buffers, Deoxyguanosine, Female, Head and Neck Neoplasms, Humans, Male, Middle Aged, Reference Standards, Reproducibility of Results, Sodium Chloride, Solutions, Urinalysis, Young Adult

ID: 98468072