Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine
Research output: Contribution to journal › Journal article › Research › peer-review
Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.
|Journal||Antioxidants & Redox Signaling|
|Number of pages||15|
|Publication status||Published - 20 Jun 2013|
- Adult, Artifacts, Buffers, Deoxyguanosine, Female, Head and Neck Neoplasms, Humans, Male, Middle Aged, Reference Standards, Reproducibility of Results, Sodium Chloride, Solutions, Urinalysis, Young Adult